Involvement of the adrenal glands and testis in gap junction formation via testosterone within the male rat anterior pituitary gland |
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Authors: | Eisuke Sakuma Ikuo Wada Takanobu Otsuka Kenjiro Wakabayashi Kinya Ito Tsuyoshi Soji Damon C. Herbert |
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Affiliation: | 1. Department of Functional Anatomy, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan;2. Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan;3. The Research Institute of System Sciences, Nihon Fukushi University, Handa, Aichi, Japan;4. Department of Cellular and Structural Biology, Dental School, University of Texas Health Science Center at San Antonio, Texas |
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Abstract: | We investigated the influence of testicular and adrenal androgens on the presence of gap junctions between folliculo‐stellate cells in the anterior pituitary glands of 60‐day‐old Wistar‐Imamichi strain male rats. The animals were separated into six groups: Group A served as the controls and had free access to a normal diet and water, Group B was given a normal diet and 0.9% NaCl for their drinking water as the controls of adrenalectomized groups, Group C was castrated, Group D was adrenalectomized, Group E was both castrated and adrenalectomized, and Group F was also both castrated and adrenalectomized. In addition, the animals of Group F were administered a dose of testosterone that is known to produce high physiological levels of the hormones in plasma. Five rats from each group were sacrificed 1, 2, 3, 4, 5, 6, and 7 days after their respective operation, and the anterior pituitary glands were removed and prepared for observation by transmission electron microscopy. We quantified the number of follicles and gap junctions and calculated the rate of occurrence as the ratio of the number of gap junctions existing between folliculo‐stellate cells per intersected follicle profile. Simultaneous removal of adrenal glands with castration resulted in a significantly decrease in the number of gap junctions, whereas the administration of testosterone to these rats compensated for this change. These observations indicate that the preservation of gap junctions between folliculo‐stellate cells is mainly dependent on androgens from both the testes and adrenal glands in adult male rats. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc. |
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Keywords: | adrenal gland anterior pituitary gland folliculo‐stellate cell gap junction rat |
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