Implants of polyanionic collagen matrix in bone defects of ovariectomized rats |
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Authors: | Marcelo Rodrigues Cunha Jr" target="_blank">Arnaldo Rodrigues SantosJr Gilberto Goissis Selma C Genari |
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Affiliation: | Department of Cell Biology, Institute of Biology, State University of Campinas, P.O. Box 6109, Campinas, SP 13084-971, Brazil. cunharmr@hotmail.com |
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Abstract: | In recent years, there has been a great interest in the development of biomaterials that could be used in the repair of bone
defects. Collagen matrix (CM) has the advantage that it can be modified chemically to improve its mechanical properties. The
aim of the present study was to evaluate the effect of three-dimensional membranes of native or anionic (submitted to alkaline
treatment for 48 or 96 h) collagen matrix on the consolidation of osteoporosis bone fractures resulting from the gonadal hormone
alterations caused by ovariectomy in rats subjected to hormone replacement therapy. The animals received the implants 4 months
after ovariectomy and were sacrificed 8 weeks after implantation of the membranes into 4-mm wide bone defects created in the
distal third of the femur with a surgical bur. Macroscopic analysis revealed the absence of pathological alterations in the
implanted areas, suggesting that the material was biocompatible. Microscopic analysis showed a lower amount of bone ingrowth
in the areas receiving the native membrane compared to the bone defects filled with the anionic membranes. In ovariectomized
animals receiving anionic membranes, a delay in bone regeneration was observed mainly in animals not subjected to hormone
replacement therapy. We conclude that anionic membranes treated with alkaline solution for 48 and 96 h presented better results
in terms of bone ingrowth. |
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