| 鸡卵黄中水溶性成分(WSF)与卵磷脂的分离及提取纯化 |
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| 引用本文： | 宋宏新. 鸡卵黄中水溶性成分(WSF)与卵磷脂的分离及提取纯化[J]. 陕西科技大学学报, 2003, 21(4): 6-9 |
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| 作者姓名： | 宋宏新 |
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| 作者单位： | 陕西科技大学生命科学与工程学院 陕西 |
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| 摘 要： | 将高免卵黄液用水稀释10倍，冻融后经微滤分离出卵黄水溶性成分(WSF)，分离WSF后的卵黄用溶剂法提取纯化卵磷脂。研究了氯仿-甲醇提取和丙酮-乙醚-乙醇纯化工艺，确定了主要参数，产品用硅胶TLC分析表明，所提取的卵磷脂纯度较高，达到了对鸡卵黄的综合利用。
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| 关 键 词： | 卵黄 水溶性成分 卵磷脂 分离 提取 纯化 鸡蛋 综合利用 |
| 文章编号： | 1000-5811(2003)04-0006-04 |
| 修稿时间： | 2003-03-22 |
PHOSPHATIDYLCHCLINE EXTRACTING AND PURIFYING FROM RESIDUE SEPARATED WSF OF EGG YOLK |
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| SONG Hong-xin. PHOSPHATIDYLCHCLINE EXTRACTING AND PURIFYING FROM RESIDUE SEPARATED WSF OF EGG YOLK[J]. Journal of Shaanxi University of Science & Technology(Natural Science Edition), 2003, 21(4): 6-9 |
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| Authors: | SONG Hong-xin |
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| Abstract: | Egg yolk was diluted 10 times with distilled water, then frozen and thawed. WSF(water-soluble fraction) was separated through filtered through 0. 45um cellulose acetate membrane. Organic solvent extracted phosphatidylchcline from the remains. The methods of extraction with chloroform -methanol and purification with acetone, ether and ethanol were studied. The final phosphatidylcholine sample was analyzed with TLC. |
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| Keywords: | phosphatidylchcline water-soluble fraction(WSF) IgY separation and purifiction |
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