Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay |
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| Authors: | Zhanhui Wang Suxia Zhang Nailya R Murtazina Sergei A Eremin & Jianzhong Shen |
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| Affiliation: | Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;
Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119998, Russia |
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| Abstract: | A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein‐labelled MD (tracer) was synthesised by N‐hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 μg mL?1 with an IC50 value of 0.16 μg mL?1 and a limit of detection of 0.002 μg mL?1. Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 μg g?1 levels were 82–130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable. |
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| Keywords: | Fluorescence polarisation immunoassay food control maduramicin polyclonal antibodies |
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