| Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites |
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| 作者姓名： | 夏金兰 傅金殿 聂珍媛 申丽 |
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| 作者单位： | School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China |
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| 摘 要： | The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0（NH2-PAMAM G2.0）-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au（Ⅲ） to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2. 0 plays an important role in acting as host or micro-reactor for Au（Ⅲ） before Au（Ⅲ） reduction and acting as dispersant and stabilizer for gold nanoparticles after Au（Ⅲ） reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses.
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| 关 键 词： | PAMAM 金 纳米粒子 细胞着色 光学性能 制备 水溶胺 |
| 文章编号： | 1005-9784（2005）06-0641-06 |
| 收稿时间： | 2005-04-26 |
| 修稿时间： | 2005-06-27 |
Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites |
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| XIA Jin-lan,FU Jin-dian,NIE Zhen-yuan,SHEN li.Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites[J].Journal of Central South University of Technology,2005,12(6):641-646. |
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| Authors: | XIA Jin-lan FU Jin-dian NIE Zhen-yuan SHEN li |
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| Abstract: | The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0(NH2-PAMAM G2.0)-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au(Ⅲ) to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 plays an important role in acting as host or micro-reactor for Au(Ⅲ) before Au(Ⅲ) reduction and acting as dispersant and stabilizer for gold nanoparticles after Au(Ⅲ) reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses. |
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| Keywords: | polyamidoamine dendrimer nanocomposites gold nanoparticles optical properties cell staining |
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