| 链霉菌9-1-1产几丁质酶的发酵条件研究 |
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| 引用本文： | 奚家勤,尹启生,宋纪真,周汉平,魏春阳. 链霉菌9-1-1产几丁质酶的发酵条件研究[J]. 中国烟草学报, 2008, 14(Z1) |
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| 作者姓名： | 奚家勤 尹启生 宋纪真 周汉平 魏春阳 |
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| 作者单位： | 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号,450001 |
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| 基金项目： | 中国烟草总公司郑州烟草研究院院长基金项目[042005C210]几丁质酶产生菌防治烟草黑胫病研究"的内容之一 |
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| 摘 要： | 从烟草根际土样中分离、筛选得到1株几丁质酶活性比较高的链霉菌9-1-1,为评价该菌株利用价值,对其发酵条件(碳源、氮源、初始pH、表面活性剂及发酵时间)进行了研究.结果表明:该菌株的最佳发酵条件以1%胶体几丁质为碳源,发酵液初始pH值为6.5,以1%蛋白胨为氮源,0.1%吐温80作为表面活性剂,发酵时间为96 h,接种量为1%,最高酶活达到56 U/mL.
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| 关 键 词： | 链霉菌9-1-1 几丁质酶 培养条件 |
Study on fermentation condition for producting chitinase from Streptomyces sp. 9-1-1 |
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| XI Jia-qin,YIN Qi-sheng,SONG Ji-zhen,ZHOU Han-ping,WEI Chun-yang. Study on fermentation condition for producting chitinase from Streptomyces sp. 9-1-1[J]. Acta Tabacaria Sinica, 2008, 14(Z1) |
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| Authors: | XI Jia-qin YIN Qi-sheng SONG Ji-zhen ZHOU Han-ping WEI Chun-yang |
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| Affiliation: | XI Jia-qin YIN Qi-sheng SONG Ji-zhen ZHOU Han-ping WEI Chun-yang Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 45001,China |
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| Abstract: | Streptomyces sp.9-1-1 which demonstrates high chitinase activity was isolated and screened from rhizosphere of to- bacco.In order to evaluate its value,the activity of chitinase was tested in different culture conditions,including carbons source,nitrogen source,initial pH,suffactant,fermentation time.Results indicated that the highest activity of chitinase was 56U/mL using 1% chitin as carbons source in medium and initial pH of fermentation fluid was 6.5 with 1% peptone as nitro- gen,0.1% Tween-80 as a surf... |
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| Keywords: | Streptomyces sp chitinases fermentation condition |
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