UTILIZATION OF PRETREATED MOLASSES FOR SERINE ALKALINE PROTEASE PRODUCTION WITH RECOMBINANT BACILLUS SPECIES |
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Authors: | Gü zide alik Nur Pehl
van Nerm
Kalender Tun er H.
zdamar Pinar alik |
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Affiliation: | a Department of Chemical Engineering, and Industrial Biotechnology Department of Biotechnology Research Center, Ankara University, Ankara, Turkey.b Department of Chemical Engineering, METU, Ankara, Turkey. |
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Abstract: | Recombinant Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis , and Bacillus licheniformis were used for the production of serine alkaline protease (SAP) utilizing chemically and/or physically pretreated molasses. The highest enzyme activity was obtained with r- Bacillus subtilis , with the complex medium involving physically treated molasses having 20 kg m -3 initial sucrose concentration in small-scale, agitation- and heating rate-controlled bioreactors at t=63 h. Effects of oxygen transfer were investigated in 3.5 dm 3 laboratory bioreactors under two different agitation rates with r- B. subtilis . Increase in the oxygen transfer rate increased the observed activity and caused the cultivation time of maximum activity shift to the earlier stages of the fermentation. At Q/V=0.5 vvm and N=750 min -1 , SAP activity reached 2250U cm -3 at t=36 h. |
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Keywords: | Serine Alkaline Protease Fermentation Utilization Of Molasses Oxygen Transfer Effects |
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