| 密度梯度分离纯化/免疫荧光技术检测饮用水中“两虫” |
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| 引用本文： | 张冬青,李红岩,李栋,于建伟,刘志斌,杨敏. 密度梯度分离纯化/免疫荧光技术检测饮用水中“两虫”[J]. 中国给水排水, 2009, 25(2) |
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| 作者姓名： | 张冬青 李红岩 李栋 于建伟 刘志斌 杨敏 |
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| 作者单位： | [1]中国科学院生态环境研究中心饮用水科学与工程中心,北京100085 [2]辽宁工程技术大学资源与环境工程学院,辽宁阜新123000 |
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| 基金项目： | 国家自然科学基金资助项目(50778171,50621804) |
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| 摘 要： | 采用膜溶解、密度梯度分离纯化结合免疫荧光染色法对饮用水中的“两虫”（隐孢子虫和贾第鞭毛虫）进行定性与定量检测，以大幅降低检测成本，简化操作。结果表明，使用Percoll-蔗糖介质可取得良好的分离纯化效果，其卵囊和孢囊初始回收率分别为56％和35％，远高于EPA1623方法的要求。利用该方法对河网型水源水及水厂滤后水进行了检测，发现10L水源水中有1～3个隐孢子虫和0～2个贾第鞭毛虫，但在50L滤后水中均未检出“两虫”。
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| 关 键 词： | 隐孢予虫 贾第鞭毛虫 密度梯度分离 免疫荧光检测 饮用水 |
Detection of Cryptosporidium and Giardia in Drinking Water Using Immunofluorescence Assay and Density Gradient Separation |
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| ZHANG Dong-qing,,LI Hong-yan,LI Dong,YU Jian-wei,LIU Zhi-bin,YANG Min. Detection of Cryptosporidium and Giardia in Drinking Water Using Immunofluorescence Assay and Density Gradient Separation[J]. China Water & Wastewater, 2009, 25(2) |
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| Authors: | ZHANG Dong-qing LI Hong-yan LI Dong YU Jian-wei LIU Zhi-bin YANG Min |
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| Affiliation: | 1. Research Center for Eco-Environmental Sciences;Chinese Academy of Sciences;Beijing 100085;China;2. College of Resource and Environmental Engineering;Liaoning Technical University;Fuxin 123000;China |
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| Abstract: | The qualitative and quantitative detection of Cryptosporidium and Giardia in drinking water was performed using membrane dissolution,density gradient separation and immunofluorescence assay to reduce the detection cost and simplify the operation procedures. The results show that when using percoll-sucrose as the centrifugal medium,the recovery rates of oocysts and cysts are 56% and 35% respectively,which is much higher than the requirement of EPA1623 method. The new method is used to detect Cryptosporidium ... |
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| Keywords: | Cryptosporidium Giardia density gradient separation immunofluorescence assay drinking water |
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