D-Peptide and D-Protein Technology: Recent Advances,Challenges, and Opportunities** |
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| Authors: | Alexander J Lander Dr Yi Jin Dr Louis Y P Luk |
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| Affiliation: | 1. School of Chemistry, Cardiff University, Main Building, Park Place, Cardiff, CF10 3AT UK;2. Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN UK |
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| Abstract: | Total chemical protein synthesis provides access to entire D-protein enantiomers enabling unique applications in molecular biology, structural biology, and bioactive compound discovery. Key enzymes involved in the central dogma of molecular biology have been prepared in their D-enantiomeric forms facilitating the development of mirror-image life. Crystallization of a racemic mixture of L- and D-protein enantiomers provides access to high-resolution X-ray structures of polypeptides. Additionally, D-enantiomers of protein drug targets can be used in mirror-image phage display allowing discovery of non-proteolytic D-peptide ligands as lead candidates. This review discusses the unique applications of D-proteins including the synthetic challenges and opportunities. |
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| Keywords: | chemical protein synthesis chemical ligation D-proteins mirror-image proteins peptides proteins protein engineering |
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