Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion** |
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| Authors: | Dr Joscha Meiers Jan Dastbaz Dr Sebastian Adam Sari Rasheed Dr Susanne H Kirsch Dr Peter Meiser Prof?Dr Peter Gross Prof?Dr Rolf Müller Prof?Dr Alexander Titz |
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| Affiliation: | 1. Chemical Biology of Carbohydrates (CBCH), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123 Saarbrücken, Germany;2. Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124 Braunschweig, Germany;3. URSAPHARM Arzneimittel GmbH, 66129 Saarbrücken, Germany;4. Hochschule Kaiserslautern, Protein Chemistry Group, 66953 Pirmasens, Germany |
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| Abstract: | The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar KD in a carbohydrate-dependent fashion. |
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| Keywords: | bromelain carbohydrates lectin mannose SARS-CoV-2 |
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