Biomechanics of the optic nerve head and peripapillary sclera in a mouse model of glaucoma |
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| Authors: | Arina Korneva Elizabeth C. Kimball Joan L. Jefferys Harry A. Quigley Thao D. Nguyen |
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| Affiliation: | 1.Glaucoma Center of Excellence, Wilmer Eye Institute, John Hopkins University, Baltimore, MD, USA;2.Department of Mechanical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA;3.Department of Ophthalmology, The Johns Hopkins University, Baltimore, MD 21218, USA;4.Department of Materials Science, The Johns Hopkins University, Baltimore, MD 21218, USA |
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| Abstract: | The deformation of the mouse astrocytic lamina (AL) and adjacent peripapillary sclera (PPS) was measured in response to elevated intraocular pressure. We subjected explanted mouse eyes to inflation testing, comparing control eyes to those 3 days and 6 weeks after induction of ocular hypertension (OHT) via ocular microbead injection. Laser scanning microscopy was used with second harmonic generation to image the collagenous PPS and two-photon fluorescence to image transgenic fluorescent astrocytes in the AL. Digital volume correlation was applied to calculate strains in the PPS and AL. The specimen-averaged strains were biaxial in the AL and PPS, with greater strain overall in the x- than y-direction in the AL and greater strain in the θ- than the r-direction in the PPS. Strains increased after 3-day OHT, with greater strain overall in the 3-day AL than control AL, and greater circumferential strain in the 3-day PPS than control PPS. In the 6-week OHT eyes, AL and PPS strains were similar overall to controls. This experimental glaucoma model demonstrated a dynamic change in the mechanical behaviour of the AL and PPS over time at the site of neuronal injury and remodelling in glaucoma. |
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| Keywords: | glaucoma optic nerve head peripapillary sclera digital volume correlation mechanical testing mouse |
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