Affiliation: | 1. Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy;2. Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini, 5, 80131 Naples, Italy;3. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 Let Vladivostoku, 690022 Vladivostok, Russia;4. Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shen-Lung Road, Ilan, 26099 Taiwan;5. Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126 Naples, Italy
Task Force on Microbiome Studies, University of Naples Federico II;6. Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via S. Pansini, 5, 80131 Naples, Italy
Task Force on Microbiome Studies, University of Naples Federico II
CEINGE-Biotecnologie Avanzate Franco Salvatore s.c.ar.l., Via G. Salvatore, 436, 80131 Naples, Italy |
Abstract: | Marine bacteria, which are often described as chemical gold, are considered an exceptional source of new therapeutics. Considerable research interest has been given to lipopolysaccharides (LPSs), the main components of the Gram-negative outer membrane. LPS and its lipid A portion from marine bacteria are known to exhibit a tricky chemistry that has been often associated with intriguing properties such as behaving as immune adjuvants or anti-sepsis molecules. In this scenario, we report the structural determination of the lipid A from three marine bacteria within the Cellulophaga genus, which showed to produce an extremely heterogenous blend of tetra- to hexa-acylated lipid A species, mostly carrying one phosphate and one D-mannose on the glucosamine disaccharide backbone. The ability of the three LPSs in activating TLR4 signaling revealed a weaker immunopotential by C. baltica NNO 15840T and C. tyrosinoxydans EM41T, while C. algicola ACAM 630T behaved as a more potent TLR4 activator. |