Pyrophosphatidic acid in mushrooms |
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| Authors: | Sugai Akihiko Itoh Toshihiro Kaneko Hiroshi Kinjo Noriko Muramatsu Toshio |
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| Affiliation: | (1) Division of Chemistry, School of General Studies, Kitasato University, 228 Kifasato, Sagamihara, Kanagawa, Japan;(2) Laboratory of Biology, Department of General Education, Tokyo Medical and Dental University, Kohnodai, Ichikawa, 272 Chiba, Japan;(3) Laboratory of Chemistry, Department of General Education, Tokyo Medical and Dental University, Kohnodai, Ichikawa, 272 Chiba, Japan |
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| Abstract: | The distribution of pyrophosphatidic acid in mushrooms was investigated. Total lipids of mushrooms were extracted from their fruit body, and the pyrophosphatidic acid fraction was isolated by DEAE-silica gel column and silicic acid column chromatography. The presence of pyrophosphatidic acid was tested by thin layer chromatography. Of the 43 species of mushrooms examined, the acid was found in 30. The pyrophosphatidic acid was identified by chromatography, IR spectroscopy and quantitative degradation with aqueous pyridine to produce phosphatidic acid. Chemically synthesized pyrophosphatidic acid was used as standard. |
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