| 氢氧化钡处理-分散液液微萃取结合气相色谱-串联质谱法测定肉制品中9种N-亚硝胺 |
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| 引用本文： | 李芳芳,梁秀清,王艳丽,陈倩倩,鞠 香,陈克云,李 洁,田其燕,刘艳明. 氢氧化钡处理-分散液液微萃取结合气相色谱-串联质谱法测定肉制品中9种N-亚硝胺[J]. 食品安全质量检测学报, 2023, 14(4): 137-144 |
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| 作者姓名： | 李芳芳 梁秀清 王艳丽 陈倩倩 鞠 香 陈克云 李 洁 田其燕 刘艳明 |
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| 作者单位： | 山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院,山东省食品药品检验研究院 |
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| 基金项目： | 山东省药品监督管理局科研项目(SDNMPAFZLS202202)、山东省食品药品检验研究院科研项目(SDIFDC-KY-2021011) |
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| 摘 要： | 目的 建立氢氧化钡碱溶液-分散液液微萃取结合气相色谱-串联质谱法(gaschromatography-tandem massspectrometry,GC-MS/MS)同时测定肉制品中9种N-亚硝胺类化合物的分析方法。方法 肉制品样品在80℃水浴中经氢氧化钡碱溶液皂化,去除油脂干扰,进一步将N-亚硝胺提取到水溶液中,再加入甲醇(分散剂)和二氯甲烷(萃取剂)进行高效萃取富集,在多反应监测模式下分析,稳定同位素内标法定量。对影响萃取效率的因素进行考察,如样品提取步骤中的皂化条件,无机盐、萃取剂和分散剂的种类及体积等。通过优化色谱、质谱参数和前处理条件,获得最佳检测结果。结果 除了N-亚硝基二甲胺(N-nitrosodimethylamine, NDMA)的线性范围为2.5～50.0 ng,其他8种N-亚硝胺类化合物在1.0～50.0 ng范围内线性关系良好, 9种N-亚硝胺类化合物的相关系数(r2)范围为0.9954～0.9999,方法检出限和定量限分别为0.1～0.2μg/kg和0.2～0.5μg/kg。在火腿、五香鸡肉和牛肉样品中分别进行0.5、5.0μg/kg浓度水平的加标实验,所有...
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| 关 键 词： | 碱液处理 分散液液微萃取 气相色谱-串联质谱法 肉制品 N-亚硝胺 |
| 收稿时间： | 2022-12-09 |
| 修稿时间： | 2023-02-08 |
Determination of 9 kinds of N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry with barium hydroxide treatment followed dispersive liquid-liquid microextraction |
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| LI Fang-Fang,LIANG Xiu-Qing,WANG Yan-Li,CHEN Qian-Qian,JU Xiang,CHEN Ke-Yun,LI Jie,TIAN Qi-Yan,LIU Yan-Ming. Determination of 9 kinds of N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry with barium hydroxide treatment followed dispersive liquid-liquid microextraction[J]. Journal of Food Safety & Quality, 2023, 14(4): 137-144 |
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| Authors: | LI Fang-Fang LIANG Xiu-Qing WANG Yan-Li CHEN Qian-Qian JU Xiang CHEN Ke-Yun LI Jie TIAN Qi-Yan LIU Yan-Ming |
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| Affiliation: | Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China,Shandong Institute for Food and Drug Control; Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes;Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101;China |
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| Abstract: | |
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| Keywords: | alkali solution treating dispersive liquid-liquid microextraction gas chromatography-tandem mass spectrometry meat products N-nitrosamines |
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