热带雨林环境中涂镀层表面的真菌群落研究

目的 研究热带雨林大气环境中5种常用涂(镀)层钢板表面的菌群分布及其变化规律,并深入讨论真菌对涂镀层材料的破坏作用.方法 对聚酯涂层、氟碳涂层、含铬耐指纹涂层、无铬耐指纹涂层和热镀锌板在内的5种材料,在西双版纳大气试验站进行2年的大气暴晒实验,干季和雨季共进行4次菌种采集.结合传统的培养法和现代分子生物学的方法,鉴定涂...

Study on Fungi Community of Coating Surface in Tropical Rainforest

In this study, our object is to investigate the fungal community structure and diversity on different coating materials in the tropical rainforest, and to discuss the corrosion or biodegradation effect of fungi.Five materials including polyester coating, fluorocarbon coating, Cr-containing anti-fingerprint coating, Cr-free anti-fingerprint coating and hot-dip galvanized sheet were exposed 2 years at Xishuangbanna atmosphere test station. We collected the fungi from the materials in dry and rainy seasons for 4 times, which were cultured by traditional culture methods and identified by molecular biology methods. The dominant genera were statistically analyzed. Scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) were used to study the corrosion behavior of molds on the coatings. Finally, 154 strains of fungi were isolated and purified. Of them, 37 genera in 10 orders of 3 classes of Ascomycota and 12 genera in 6 orders of 3 classes of Basidiomycota were identified by ITS rDNA sequence analysis. The dominant genera based on statistics were Daldinia, Xylaria, Aspergillus, Talaromyces, Curvularia, Coprinellus, Alternaria, Periconia, Coprinopsis, and Pithomyces. The life activities of the dominant genera, especially Xylaria, Curvularia, and Aspergillus may have a strong correlation with the coatings deterioration process, which can be used in the indoor fungus test.