有核细胞光散射强度分布的动态特性

针对核式形体细胞微粒,基于双椭球核式(DCEM)模型,应用数值计算技术分别讨论了细胞在大小变化、胞质厚度变化、核质大小变化、细胞形体变化、折射率变化、入射角变化情况下对其光散射强度分布所产生的影响,并建立相应的模型进行数值计算和三维拟合。得到了有核细胞光散射强度分布与有核细胞相关物理特征量变化下的动态响应关系及其影响特征。此外,对有核细胞在形体和入射角连续变化情况下,光散射强度所受的影响进行了数值拟合,并对拟合效果进行了误差估算。促进了真实形体细胞测量修正理论的完善和微扰测量理论的实际应用,为单细胞的光学检测和鉴别技术的提高提供了理论依据。

Dynamic Characters of Light Scattering Intensity Distribution for a Nuclear-Cell

Considering of a nuclear cell particles based on the double concentric ellipsoids model(DCEM),the numerical analysis technique is applied to discuss the scattering intensity affection of the cell separately,such as,in the variety of size,cytoplast,karyoplasms,body,refractive index,and an angel of incidence.A dynamic relationship and characters of responded scattering intensity distribution and related varying characteristic parameters of a nuclear cell are gotten after calculating and three-dimensional(3D) fitting upon the modified motel.Besides,scattering intensity distribution affected by its body and varying incidence angles is fitted and the error calculating of fitting effecting is estimated.These promote the perfecting of modifying theory for real cell inspecting and available applying of weak-disturbance modifying measuring theory;these also provide a theory foundation for improving of measuring technique and distinguish technique of cells.