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低能重离子束注入小麦种子诱导根尖细胞有丝分裂畸变的研究
引用本文:顾月华,王圣兵,罗建平,陈为钧.低能重离子束注入小麦种子诱导根尖细胞有丝分裂畸变的研究[J].核技术,2000,23(8):587-592.
作者姓名:顾月华  王圣兵  罗建平  陈为钧
作者单位:中国科学技术大学生命科学学院 合肥230026
基金项目:国家自然科学基金,19890300,
摘    要:比较研究了低能重离子束注入小麦干种子和萌动的种子对种子发芽及根尖细胞有丝分裂畸变的影响。结果表明,离子注入对萌动的种子的发芽有明显的影响,而对干种子的发芽影响不明显。在一定剂量范围内(0-6×1016/cm2),N+注入诱导的萌动种子的有丝分裂畸变率略高于干种子,并且随辐射剂量的升高而升高;但超过一定的剂量(>6×1016/cm2),无论是干种子还是萌动种子的有丝分裂畸变率达到一定程度的饱和。就实验结果对N+离子束注入诱变的机理进行了一些探讨。

关 键 词:离子注入  小麦  种子  根尖细胞  诱变育种
修稿时间:1999年5月25日

Study on the mitotic aberrations of root-tip cells in wheatinduced by the implantation of low energy heavy ion beams
GU Yuehua,WANG Shengbing,LUO Jianping,Chen Weijun.Study on the mitotic aberrations of root-tip cells in wheatinduced by the implantation of low energy heavy ion beams[J].Nuclear Techniques,2000,23(8):587-592.
Authors:GU Yuehua  WANG Shengbing  LUO Jianping  Chen Weijun
Abstract:The effects of N ion beam implantation on seed germinating rate and root-tip mitotic aberration rate for dry and sprouting seeds are compared. Results show that N ion beam implantation brought about obvious effects on sprouting seeds but little effects on dry seeds. Within the range of dose (0- 6x 1016/cm2), the mitotic aberrations of sprouting seeds induced by N ion beam implantation are higher than those of dry seeds, and they all increase with the increasing implantation dose; but when the implantation dose is higher than 6 x 1016/cm2, they all show some degree of saturation. Possible mechanism of aberration effects induced by N ion beam implantation is briefly discussed.
Keywords:Ion beam implantation  Wheat  Dry seeds and sprouting seeds  Nuclear aberration  Chromosome aberration  Mitotic aberration  Mutagenesis mechanism  
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