Green fluorescent protein as a reporter to monitor gene expression and food colonization by Aspergillus flavus |
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| Authors: | W Du Z Huang JE Flaherty K Wells GA Payne |
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| Affiliation: | Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695-7616, USA. |
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| Abstract: | Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants. |
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