| 饲养细胞用于挽救濒死的珍稀贴壁细胞 |
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| 引用本文： | 李利,杨屹,张宇,邵丽军,谷丽娟,金立杰. 饲养细胞用于挽救濒死的珍稀贴壁细胞[J]. 中国生物制品学杂志, 2007, 20(2): 136-138,141 |
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| 作者姓名： | 李利 杨屹 张宇 邵丽军 谷丽娟 金立杰 |
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| 作者单位： | 长春生物制品研究所疫苗一室 长春130062(李利，杨屹，张宇，谷丽娟，金立杰)，潍坊医学院预防医学系 潍坊260000(邵丽军) |
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| 摘 要： | 目的挽救濒于死亡的珍稀贴壁细胞。方法将濒于死亡的RINm5F细胞接种到一定量体外培养的小鼠腹腔细胞中,经过一段时间的培养,待RINm5F细胞逐渐长成岛状时,传代并再连续添加2次饲养细胞培养后,传2代,冻存。结果濒于死亡的RINm5F细胞与饲养细胞共培养,随着时间的延长,细胞生长速度加快,饲养细胞逐渐死亡,再经2次与饲养细胞共培养,细胞已恢复正常生长,冻存后复苏生长状态良好。结论添加饲养细胞的方法可用于挽救濒于死亡的细胞。
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| 关 键 词： | 饲养细胞 贴壁细胞 挽救 |
| 文章编号： | 1004-5503（2007）02-136-04 |
| 收稿时间： | 2006-04-25 |
| 修稿时间： | 2006-04-25 |
Rescue of Dying Adherent Cells by Feeder Cells |
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| LI Li , YANG Yi ,ZHANG Yu, et al. Rescue of Dying Adherent Cells by Feeder Cells[J]. Chinese Journal of Bilogicals, 2007, 20(2): 136-138,141 |
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| Authors: | LI Li YANG Yi ZHANG Yu et al |
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| Affiliation: | Changchun Institute of Biological Products, Changchun 130062, China |
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| Abstract: | Objective To rescue the dying adherent cells.Methods Inoculate the dying RINm5F cells into a certain amount of mouse celiac cells and culture for a certain time.When a dense monolayer was formed,the RINm5F cells was subcultured and added with feeder cells for 2 times,then stored lyophilized.Results Both the growth rate of dying RINm5F cells and the mortality rate of feeder cells increased as the time for co-culture goes on.After further co-culture for 2 times,the RINm5F grew normally.After reconstitution,the freeze-dried RINm5F cells grew well.Conclusion The addition of feeder cells might be used for rescuing the dying adherent cells. |
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| Keywords: | Feeder cells Adherent cells Rescue |
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