不同处理工艺对玫瑰花中抗氧化物质含量及抗氧化活性的影响

目的 研究不同处理工艺对平阴玫瑰中总黄酮、芦丁、总多酚含量及抗氧化活性的影响。方法 对干花直接提取、干花超微粉碎提取以及鲜花冷冻干燥提取3种不同处理工艺的平阴玫瑰进行水提，采用分光光度法测定水提液中总黄酮、总多酚的含量，高效液相色谱法测定水提液中芦丁含量，并对3种不同处理工艺的水提液进行1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除、羟自由基清除以及超氧阴离子清除能力的研究。结果 3种不同工艺处理的玫瑰花总黄酮含量、芦丁含量、总多酚含量及抗氧化活性均为：鲜花冷冻干燥>干花超微粉碎>干花直接提取。结论 经冷冻干燥处理后的平阴玫瑰抗氧化性物质含量及抗氧化活性均有升高，为玫瑰花抗氧化应用提供依据。

Effects of different treatment processes on antioxidant content and antioxidant activity in rose

Objective To study the effects of different treatment processes on the content of total flavonoids, rutin, total polyphenols and antioxidant activity in Pingyin Rosa rugosa. Methods The aqueous extraction of Pingyin rosa rugosa was carried out with three different treatment processes: direct extraction of dried flowers, ultra-microcrushing of dried flowers, and freeze-drying of flowers. The contents of total flavonoids and total polyphenols in the aqueous extract were determined by spectrophotometry, and the content of rutin in the aqueous extract was measured by high-performance liquid chromatography (HPLC), and the aqueous extracts of three different treatment processes were investigated for the radical scavenging, hydroxyl radical scavenging, and superoxide anion scavenging abilities of 1,1-diphenyl-2-picrylhydrazyl, DPPH. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, and superoxide anion scavenging were investigated in the aqueous extracts of three different treatment processes. Results The results showed that the total flavonoid content, rutin content, total polyphenol content and antioxidant activity of rosa rugosa treated by 3 kinds of different processes were freeze-dried flowers>dried flowers were ultrafinely crushed>dried flowers were directly extracted. Conclusion The content of antioxidant substances and antioxidant activity of Pingyin rosa tugosa after freeze-drying treatment isincreased, which provides a basis for the application of antioxidant in rosa rugosa.