Cloning of Aspergillus oryzae Aovps5 gene,homologous to vacuolar protein sorting associated gene VPS5 and construction of the disruptant |
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Authors: | Chikara Tokunaga Chiaki Saitoh Katsuhiko Kitamoto |
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Affiliation: | 1 Kyowa Hakko Food Specialties Co., Ltd., Tokyo Marketing Center 34-2, Hakozaki-Cho, Nihonbashi Chuo-ku, Tokyo 103-0015, Japan;2 Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan |
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Abstract: | Aovps5 gene was isolated from Aspergillus oryzae as a homologue to S. cerevisiae VPS5 gene which encodes a polypeptide consisting of 451 amino acids that is nearly 32% homologous to Vps5p. Three Aovps5 gene disruptants were generated and they showed higher activity of tripeptidyl peptidase, which is mainly detected in vacuoles, in their culture medium. Higher amount of nitrogenous constituent was found in the filtrate of wheat gluten degraded by addition of culture medium of these disruptants than that of wild type strain. These results suggest that disruption of Aovps5 may contribute to production of fermented foods. |
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Keywords: | Aspergillus oryzae Vacuolar protein sorting VPS5 Sorting nexin1 Degradation Seasoning |
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