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Polyunsaturated fatty glyceride syntheses by microbial lipases
Authors:Kyoichi Osada  Koretaro Takahashi  Mutsuo Hatano
Affiliation:(1) Laboratory of Food Chemistry, Faculty of Fisheries, Hokkaido University, 041 Hokadate, Japan;(2) Taiyo Central R&D Institute, Taiyo Fishery Co. Ltd., Tsukishima Chuoku, 104 Tokyo, Japan
Abstract:The degree of glyceride syntheses by lipase TOYO (Chromobacterium viscosum) and lipase OF (Candida cylindracea) using individual free fatty acids C18∶1, C18∶2, C18∶3, C18∶4, C20∶4, C20∶5 and C22∶6 were compared. Lipase TOYO incorporated each of the fatty acids into glycerol at levels of greater than 89%. Lipase OF incorporated most of the fatty acids at levels above 70% (docosahexaenoic acid incorporation was 63%). It was concluded that these two lipases are feasible for producing glycerides from unsaturated fatty acids.
Keywords:Fatty acid  glyceride  lipase  polyunsaturated  triglyceride
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