The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation |
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| Authors: | Jhaleh Amirian Nguyen Thuy Ba Linh Young Ki Min Byong‐Taek Lee |
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| Affiliation: | 1. Department of Regenerative Medicine, College of Medicine, Soonchunhyang University 366‐1, Ssangyong‐Dong, Republic of Korea;2. Department of Regenerative Medicine, Institute of Tissue Regeneration, Soonchunhyang University 366‐1, Ssangyong‐Dong, Republic of Korea;3. Department of Physiology, College of Medicine, Soonchunhyang University 366‐1, Ssangyong‐dong, Republic of Korea |
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| Abstract: | A composite scaffold of gelatine (Gel)‐pectin (Pec)‐biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein‐2 (BMP‐2) and vascular endothelial growth factor (VEGF) were loaded into the Gel‐Pec‐BCP hydrogel scaffolds by freeze‐drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X‐ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP‐2 and VEGF into the Gel‐Pec‐BCP hydrogel scaffolds. In addition MC3T3‐E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP‐2 and VEGF on cell viability and proliferation. The Gel‐Pec‐BCP scaffolds loaded with VEGF and BMP‐2 demonstrated more cell spreading and proliferation compared to those of the Gel‐Pec‐BCP scaffolds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41241. |
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| Keywords: | biocompatibility biomaterials porous materials |
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