番茄晚疫病病菌培养条件筛选

本试验选用佳木斯地区采集的番茄晚疫病病菌，分别在不同培养基、碳源、氮源、Ph、温度及光照时间的条件下培养，观察并记录菌落直径、产孢量及萌发率．来筛选番茄晚疫病病菌最佳培养条件．结果表明：黑麦培养基最适宜菌丝生长及产孢，以蔗糖做碳源最好，甘露糖最差；氮源以氨基丙酸最好，胱氨酸最差．孢子囊萌发的最适温度为15℃，低于5℃和高于35℃都不能萌发；光照抑制孢子囊的萌发，pH为7时孢子囊萌发率最高．

Screening the Culture Conditions for Phytophthora Infestans on Tomato

In this experiment, the phytophthora infestans of tomato collected from Jiamusi. Scnlture Phytophthora infestans was cultured on several medium, C source, N source, pH, temperature and light to select the best Culture Conditions. The results show that BSA was the best medium. The light held up the growth of mycelium and sporangium, and the dark generated the promoting effect. The pathogen could make full use many kinds of car- hohydra tes. The sucrose was the best C source and mannose was the worst C source. Alanine was the best N solace while cystine was the worst one. The temperature for mycelial growth and sporulation was between 10℃ and 30℃ ,and the optimum temperature was 20℃. The proper pH values ranged from 5 to 9. The optimum temperature was 15℃. The light had no effect on the sporangium germination, and the proper pH value was 7.