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雪莲果的组织培养
引用本文:刘德江,;申健,;李虎林,;丛慧颖. 雪莲果的组织培养[J]. 佳木斯工学院学报, 2009, 0(2): 306-308
作者姓名:刘德江,  申健,  李虎林,  丛慧颖
作者单位:[1]佳木斯大学生命科学学院,黑龙江佳木斯154007; [2]延边大学,延吉133400
基金项目:教育部博士留学回国基金(2002(247)).
摘    要:为了得到长势一致的雪莲果幼苗,采用组织培养的方法进行快速繁殖.结果表明:培养基MS+2,4-D(2.0mg/L)+NAA(1.0mg,L)适合愈伤组织的诱导;培养基MS+BA(2.0mg/L)+NAA(0.20mg/L)适合芽的诱导;培养基1/2MS+NAA(1.0mg/L)+IBA(0.5mg/L)适合再生植株生根.田园土:粗沙:腐殖土=1:1:1对组培苗的生长最有利.

关 键 词:雪莲果  组织培养  试管苗

Tissue Culture of Yacon
Affiliation:LIU De - Jiang , SHEN Jian, LI Hu - Lin, CONG Hui - Ying (1.College of Life Science, Jiamusi University, Jiamusi 154007, China; 2.Yanbian University, Yanji 133400, China)
Abstract:Tissue culture technology was used to obtain uniform seedlings of Yacon. The results showed: MS + 2, 4-D(2.0mg/L) + NAA(1.0mg/L) was the best to induce the callus of leaves; MS + BA(2.0mg/L) + NAA(0. 20mg/L) was the best to induce buds of stems; 1/2MS + NAA(1.0mg/L) + IBA(0.5mg/L) was the best to induce the roots of regeneration plants. It was the most feasible for the growth of seedling of tissue culture when the ratio of glebe soil, sand and mold is 1 : 1 : 1 .
Keywords:Yacon  tissue culture  test - tube seedlings
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