SSEL,a selective enrichment broth for simultaneous growth of Salmonella enterica,Staphylococcus aureus,Escherichia coli O157: H7, and Listeria monocytogenes |
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| Authors: | Yang Qu Yalong Bai Yanhong Liu Changyan Zhou Xiujuan Zhou Donglai Zhang Chunlei Shi Yujuan Suo |
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| Affiliation: | 1. Institute of Agro-food Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China;2. Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, Pennsylvania, USA;3. MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China |
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| Abstract: | Salmonella enterica, Staphylococcus aureus, Escherichia coli O157: H7, and Listeria monocytogenes may contaminate similar types of food and cause foodborne disease. The objective of this study was to develop a selective enrichment broth for simultaneous enrichment of Salmonella enterica, Staphylococcus aureus, Escherichia coli O157: H7, and Listeria monocytogenes (SSEL) using nalidixic acid, acriflavine, lithium chloride, and sodium cholate as selective agents. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully support the simultaneous enrichment of target pathogens with similar growth rates and inhibit the growth of most nontarget bacteria effectively. The enrichment effect of SSEL was confirmed by artificial contamination test coupled with multiplex PCR. In summary, SSEL has been shown to be a promising multiplex selective enrichment broth for the detection of the four pathogens on a single-assay platform. |
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