超高效液相色谱-四极杆/静电场轨道阱高分辨质谱快速测定乳及乳制品中21种真菌毒素

建立利用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱联用技术同时测定乳及乳制品中21种真菌毒素的方法.乳及乳制品经酸化乙腈提取后,采用Captiva-EMR Lipid净化柱净化,以C18色谱柱进行色谱分离,质谱采用全扫描/数据依赖的二级扫描模式(full scan data-dependent MS/MS acqu...

Rapid Determination of 21 Mycotoxins in Milk and Dairy Products by Ultra Performance Liquid Chromatography-quadrupole/orbitrap High Resolution Mass Spectrometry

A method for the simultaneous determination of 21 mycotoxins in milk and dairy products using ultra performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry technology was established. After the milk and dairy products were extracted with acidified acetonitrile, they were purified by a Captiva-EMR Lipid purification column and chromatographically separated by a C₁₈ column. The mass spectrometry adopt full scan data-dependent MS/MS acquisition mode (Full MS/ddMS2) detected in positive and negative ion modes, and quantified by external standard method. The 21 mycotoxins all had a good linear relationship within a certain concentration range, the correlation coefficients (r) were greater than 0.99, the limits of quantification were 1.0~93.8 μg/kg, and the average recoveries of all mycotoxins were 60.3%~107.0% at three different levels, and the relative standard deviations (RSD) ranged between 0.4%~14.4%. This method was used to detect commercially available powdered and liquid milk and dairy products. Aflatoxin B₂ was detected in a fruit-added yogurt sample. The method was fast, accurate, suitable for the rapid determination of multiple mycotoxins in milk and dairy products, which could be used for efficient screening of mycotoxins risks.