巴拉圭茶对高脂膳食小鼠氧化还原状态的影响

目的:探究不同剂量巴拉圭茶对高脂膳食小鼠组织氧化还原状态的影响。方法:选取6周龄C57BL/6J雄性小鼠48只，随机分为四组，对照组（ND，基础日粮），高脂组（HF，高脂日粮），高脂+低剂量巴拉圭茶组（HFL，高脂日粮，按1 g/kg剂量灌胃巴拉圭茶），高脂+高剂量巴拉圭茶组（HFH，高脂日粮，按2 g/kg剂量灌胃巴拉圭茶）。6周后处死小鼠，测定小鼠肝脏氧化还原状态指标，肝脏FoxO3a、catalase、MnSOD基因表达水平。结果:与ND组相比，HF组小鼠肝脏活性氧自由基（Reactive oxygen species，ROS）水平、丙二醛（Malondialdehyde，MDA）含量显著增加（P < 0.05），过氧化氢酶（Catalase，CAT）活性、超氧化物歧化酶（Superoxide dismutase，SOD）活性、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）活性、谷胱甘肽/氧化型谷胱甘肽（Glutathione/Oxidized glutathione，GSH/GSSG）比值、总抗氧化能力（Total antioxidant capacity，T-AOC）水平显著降低（P < 0.05），肝脏FoxO3a、catalase、MnSOD基因表达量显著下降（P < 0.05）；与HF组相比，HFL 及HFH小鼠肝脏ROS水平、MDA含量显著降低（P < 0.05），CAT活性、SOD活性、GSH-Px活性、GSH/GSSG比值、T-AOC水平显著升高（P < 0.05），肝脏FoxO3a、catalase、MnSOD基因表达量显著提高（P < 0.05）。相关性分析表明，ROS与FoxO3a、catalase、MnSOD基因表达量成极显著负相关（P < 0.01），CAT、SOD、GSH-Px活性与FoxO3a、catalase、MnSOD基因表达量成极显著正相关（P < 0.01）。结论:适量的巴拉圭茶能改善高脂膳食造成小鼠氧化还原状态失衡的现象，这与FoxO3a基因通路有一定关系。

Effects of Ilex paraguariensis on Redox Status in High-fat Diet Fed Mice

Objective: To investigate the effects of Ilex paraguariensis on redox status in high-fat diet fed mice. Methods: Forty-eight male C57BL/6J mice of six-week-old were randomly divided into 4 groups, normal diet (ND), high-fat diet (HF), high-fat diet+1 g/kg Ilex paraguariensis (HFL), high-fat diet+2 g/kg Ilex paraguariensis (HFH). The mice were sacrificed six weeks later. Hepatic redox status-related indicators were measured. The expression of FoxO3a, catalase and MnSOD genes in liver were analyzed. Results: Compared with the ND group, the levels of ROS and MDA in the liver of the HF group were increased significantly (P < 0.05), CAT, SOD, GSH-Px, GSH/GSSG values and T-AOC were reduced significantly (P < 0.05), the expression of FoxO3a, catalase and MnSOD genes in the liver were decreased significantly (P < 0.05). Compared with the HF group, the levels of ROS and MDA in the liver of the HFL and HFH groups were reduced significantly (P < 0.05), CAT, SOD, GSH-Px, GSH/GSSG values and T-AOC were increased significantly (P < 0.05), the expression of FoxO3a, catalase and MnSOD genes in the liver were increased significantly (P < 0.05). Correlation analysis showed that ROS and FoxO3a, catalase, MnSOD genes expression were significantly negative correlation (P < 0.01), CAT, SOD, GSH-Px and FoxO3a, catalase, MnSOD genes expression were significantly positive correlation (P < 0.01). Conclusion: Appropriate amount of Ilex paraguariensis can improve the redox state imbalance of mice caused by high-fat diet, which has a certain relationship with FoxO3a gene pathway.