| 茯苓渣多糖组成分析及体外抗癌、免疫活性研究 |
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| 引用本文： | 关玉婷,温思萌,冯雪,白云鹏,陈瑞瑞,沈晓勇,冯佳宁,常世敏,程鑫颖. 茯苓渣多糖组成分析及体外抗癌、免疫活性研究[J]. 食品工业科技, 2022, 43(21): 381-387. DOI: 10.13386/j.issn1002-0306.2022010168 |
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| 作者姓名： | 关玉婷 温思萌 冯雪 白云鹏 陈瑞瑞 沈晓勇 冯佳宁 常世敏 程鑫颖 |
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| 作者单位： | 1.河北工程大学生命科学与食品工程学院,河北邯郸 0560382.邯郸市天然产物与功能食品开发重点实验室,河北邯郸 0560383.晨光生物科技集团邯郸有限公司,河北邯郸 056004 |
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| 基金项目： | 河北省重点研发计划项目（18227139D）。 |
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| 摘 要： | 目的:以茯苓渣为原料,通过热水浸提茯苓多糖,研究其单糖组成及抗癌、抗炎症因子及免疫活性,为开发茯苓多糖相关产品提供了理论依据。方法:利用高效液相色谱和红外光谱对水提茯苓多糖进行单糖组成分析,采用CCK-8法对水提茯苓多糖进行抗肿瘤及免疫活性研究。结果:水提茯苓多糖的多糖含量为76%,具有明显的多糖特征吸收峰,主要由葡萄糖、甘露糖、半乳糖、岩藻糖组成,其物质的量之比为1:0.107:0.079:0.018;对胃癌细胞、乳腺癌细胞、肝癌细胞均有抑制作用,对胃癌细胞的增殖抑制效果最优（IC50=1096 μg/mL）,对小鼠脾淋巴细胞具有促进增殖作用。结论:水提茯苓多糖具有良好的体外抗癌及免疫活性。
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| 关 键 词： | 茯苓 多糖 结构表征 抗癌活性 免疫活性 |
| 收稿时间： | 2022-01-20 |
Composition Analysis,Anticancer and Immune Activities of Polysaccharides from Poria cocos Residues in Vitro |
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| GUAN Yuting,WEN Simeng,FENG Xue,BAI Yunpeng,CHEN Ruirui,SHEN Xiaoyong,FENG Jianing,CHANG Shimin,CHENG Xinying. Composition Analysis,Anticancer and Immune Activities of Polysaccharides from Poria cocos Residues in Vitro[J]. Science and Technology of Food Industry, 2022, 43(21): 381-387. DOI: 10.13386/j.issn1002-0306.2022010168 |
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| Authors: | GUAN Yuting WEN Simeng FENG Xue BAI Yunpeng CHEN Ruirui SHEN Xiaoyong FENG Jianing CHANG Shimin CHENG Xinying |
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| Affiliation: | 1.College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China2.Handan Key Laboratory of Natural Products and Functional Foods, Handan 056038, China3.Chenguang Biotech Group Co., Ltd., Handan 056004, China |
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| Abstract: | Objective: The monosaccharide composition of Poria cocos polysaccharide (PCOS) from Poria cocos residue extracted by hot water, as well as its anti-cancer, anti-inflammatory factors and immune activities were studied, providing a theoretical foundation for the development of PCOS related products. Methods: The monosaccharide composition of water-extracted PCOS was analyzed by HPLC and FTIR, and the anti-tumor and immune activities of water-extracted PCOS were studied by CCK-8 method. Results: The polysaccharide content of PCOS was 76%, mainly composed of glucose, mannose, galactose and fucose, and the molar ratio was 1:0.107:0.079:0.018, with obvious polysaccharide characteristic absorption peak. The proliferation of gastric cancer cells, breast cancer cells, and liver cancer cells was decreased, with the best inhibition impact on gastric cancer cells (IC50=1096 μg/mL), and spleen lymphocyte proliferation in mice was enhanced. Conclusion: The water-extracted PCOS has good anti-cancer and immune activities in vitro. |
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