红曲废渣发酵制备枯草芽孢杆菌微生态制剂

本研究通过单因素和响应面分析优化红曲废渣制备枯草芽孢杆菌微生态制剂的条件,对发酵过程中可溶性糖和可溶性蛋白质的含量进行追踪,以分析菌体增长的限制因素,并对该微生态制剂的储藏稳定性和体外抗氧化性进行评估.结果表明,当发酵条件为:红曲废渣浓度100 g/L,枯草芽孢杆菌接种量为10％(5 lg CFU/mL),摇床转速为2...

Production of Bacillus subtilis Microbioecologics by Fermentation of Hongqu Residue

In this study, single factor and response surface analysis were used to optimize the conditions for the production of Bacillus subtilis using Hongqu residue, the content of soluble sugar and soluble protein components during the fermentation were determined to analyze the limiting factors of bacterial growth, and the storage stability and the in vitro antioxidant activity of the microbioecologics were determined. The results showed that the highest yield of Bacillus subtilis in microbioecologics was (9.3±0.3) lg CFU/mL that was produced at 37 ℃ for 48 h with the conditions of 100 g/L Hongqu residue, and shaking speed of 220 r/min with 10% inoculation volume of 5 lg CFU/mL Bacillus subtilis. The content of soluble glucose in Hongqu residue was the main factor limiting the growth of Bacillus subtilis. When soluble sugar contents were low, the growth of Bacillus subtilis basically stopped. According to the accelerated preservation experiment, the viable count of probiotics stored at 20 ℃ for three months was 8.7 lg CFU/mL, which was higher than the criteria of microbioecologics application. The microbioecologics had strong antioxidant activity that scavenging capacity of DPPH free radical and hydroxyl free radical were 0.25 mg/mL (IC₅₀) and 0.69 mg/mL (IC₅₀) , respectively. This study proved that Hongqu residue could be used as a raw material to prepare Bacillus subtilis probiotics, which provided a practical basis for the sustainable green processing of microbial fermentation residue.