棘胸蛙肌肉水解物的制备及抗氧化活性评价

以棘胸蛙腿部肌肉为研究材料，利用木瓜蛋白酶和酸性蛋白酶两种酶，以成品率和水解度为指标，通过单因素结合响应面分析的方法优化棘胸蛙水解物的酶解工艺，同时对棘胸蛙水解物抗氧化能力进行研究。结果表明，棘胸蛙水解物经木瓜蛋白酶和酸性蛋白酶的最佳酶解条件为：在各酶最适pH和温度的条件下，料液比为1:15(g/mL)、酶解时间4 h、加酶量为2%(m/m)；该工艺条件下，棘胸蛙水解物的水解度分别为19.23%、23.51%，成品率分别为22.66%、15.32%，清除DPPH自由基的IC₅₀值分别为2.61、2.95mg/mL，清除ABTS⁺自由基的IC₅₀值分别为3.46、3.20 mg/mL，清除羟基自由基的IC₅₀值分别为8.20、9.23 mg/mL。综上，通过响应面法优化的棘胸蛙水解物酶解工艺方便可行，制备得到的水解物具有较强抗氧化活性，为棘胸蛙资源的开发提供了理论基础。

Preparation of Quasipaa spinosa Muscle Hydrolysates and Evaluation of Their Antioxidant Activity

The leg muscles of Quasipaa spinosa were enzymatically hydrolyzed using papain and acid protease to prepare hydrolysates. With the yield and degree of hydrolysis as indicators, the enzymatic hydrolysis process of Q. spinosa was optimized using single factor analysis combined with response surface methodology, and the antioxidant activity of the obtained Q. spinosa hydrolysates was evaluated. The optimal enzymatic hydrolysis conditions of Q. spinosa muscles using papain and acid protease were as follows: under the optimal pH and temperature for each enzyme, the solid-liquid ratio was 1:15, hydrolysis time was 4 h, and enzyme concentration was 2%. Under these process conditions, the degree of hydrolysis of Q. spinosa muscles obtained with papain and acid protease treatment was 19.23% and 23.51%; yield was 22.66% and 15.32%; IC50 values for DPPH free radical scavenging were 2.61 and 2.95 mg/mL; IC50 values for ABTS+ radical scavenging were 3.46 and 3.20 mg/mL; and IC50 values for hydroxyl radical scavenging were 8.20 and 9.23 mg/mL, respectively. In summary, the enzymatic hydrolysis process of Q. spinosa muscles optimized using response surface methodology is convenient and feasible, and the obtained hydrolysates exhibit potent antioxidant activity. These findings provide a theoretical basis for the development of Q. spinosa resources.