佛手果皮精油的提取工艺优化及其成分与抗氧化活性分析

本研究采用纤维素酶辅助水蒸气蒸馏提取法提取佛手果皮精油,在以酶解pH、酶添加量、酶解温度及酶解时间作为单因素分析的基础上,通过Box-Behnken响应面设计法进行提取工艺优化。利用气相色谱-质谱(Gas Chromatography-Mass Spectrometry,GC-MS)法分析提取的精油的化学组成,最后以ABTS⁺·和DPPH·清除率为指标,评价佛手果皮精油的抗氧化活性。结果表明,佛手果皮精油最佳提取工艺为:酶解pH5.2、酶添加量0.7%、酶解温度52℃、酶解时间2.1 h,此条件下精油得率为3.11%。从提取的果皮精油中共鉴定出42种化合物,其中乙酸芳樟酯的相对含量最高(14.72%),其次为d-柠檬烯(14.58%)、芳樟醇(8.89%)。抗氧化活性研究结果显示:该法提取的佛手果皮精油在试验浓度范围内具有良好的抗氧化活性,并呈现明显量效关系。当精油浓度为40 mg/mL时,其对ABTS⁺·的清除率为91.20%;浓度达70 mg/mL时,其对DPPH·的清除率达93.19%。此优化工艺精油得率高,且佛手果皮精油其可作为天然抗...

Optimization of Extraction Process of Essential Oil from Bergamot Peel and Analysis of Its Components and Antioxidant Activity

In this study, cellulase-assisted steam distillation was used to extract essential oil from bergamot peel. Based on the single factor analysis of enzymatic hydrolysis pH, enzymatic addition amount, enzymatic hydrolysis temperature and enzymatic hydrolysis time, the extraction process was optimized by using Box-Behnken response surface design method. Moreover, the chemical compositions of the extracted essential oil were analyzed by Gas chromatography-mass Spectrometry (GC-MS). Finally, the antioxidant activities of the essential oil was evaluated by the scavenging rates of ABTS+· and DPPH·. According to the research results, 3.11% yield of essential oil was achieved under the optimal extraction conditions: enzymatic hydrolysis of pH5.2, enzymatic dosage of 0.7%, enzymatic hydrolysis temperature of 52 ℃, and enzymatic hydrolysis time of 2.1 h. A total of 42 compounds were identified from the extracted peel essential oils. Among them, linalool acetate had the highest (14.72%) relative content, followed by d-limonene (14.58%) and linalool (8.89%). Besides, according to the results of antioxidant activity study, the essential oil extracted by this method presented good antioxidant activity in the experimental concentration range, and showed an obvious dose-effect relationship. The scavenging rate of ABTS+· reached 91.20% when the concentration of essential oil was 40 mg/mL; and the scavenging rate of DPPH· reached 93.19% when the concentration of essential oil was 70 mg/mL. Therefore, this optimized process has high yield of essential oil and can be developed as a natural antioxidant.