棘胸蛙肌肉营养成分季节变化分析与评价

棘胸蛙肌肉营养成分受季节变化影响显著,分析不同季节棘胸蛙肌肉营养成分的组成,旨在为后续产品深加工提供理论依据,在加工过程中可以依据产品需求选择合适季节的棘胸蛙.以春、夏、秋、冬四个季节的棘胸蛙(Quasipaa spinose)成蛙为试验对象,采用索氏提取法、凯氏定氮法和色谱法等分析其肌肉常规营养成分、氨基酸和脂肪酸组...

Analysis and Evaluation of Seasonal Changes on Nutrient Composition in Muscle of Quasipaa spinosa

The analysis of the nutritional composition of the muscle of Quasipaa spinosa in four seasons aimed to provide theoretical basis for the further processing of Quasipaa spinosa. In the processing process, Quasipaa spinosa in the appropriate season could be selected according to the product requirements. Samples of Quasipaa spinosa in four seasons were collected to analyze the differences of muscle basic nutrient composition, amino acids, fatty acids by Soxhlet extraction, Kjeldahl nitrogen determination and chromatography. The results showed that the nutrient composition varied greatly in different seasons. The contents of crude protein (24.79±0.32 g/100 g) and crude fat (1.19±0.17 g/100 g) were the highest in winter, and the contents of water and ash were the highest in autumn. However, 17 kinds of amino acids were all contained in muscles in different seasons, and the contents of lysine (8.73~10.20 g/100 g) was the highest in essential amino acids, and methionine+cysteine was the lowest. The total amino acids (TAA), essential amino acids (EAA) and flavor amino acids (DAA) were the highest in autumn. Valine and methionine+cystine were limiting amino acids, but the first and the second limiting amino acids were different in different seasons. Fatty acids were the most in autumn (18 kinds), and oleic acid (C18:1n9c) content was the highest, while linoleic acid (C18:2n6c) content was the highest in the other three seasons. The muscle nutrient composition of Quasipaa spinosa was significantly affected by seasonal changes.