牦牛胶原蛋白肽降血糖效果研究

利用小鼠的糖尿病模型，研究牦牛胶原蛋白肽的降血糖作用。通过对昆明鼠腹腔注射四氧嘧啶（200 mg/kg）制备糖尿病小鼠模型。将造模成功的小鼠随机分为正常组、模型组、阳性对照组（二甲双胍200 mg/kg）和牦牛胶原蛋白肽组（200 mg/kg），每组6只，每天灌胃给药一次，连续灌胃2周，正常组和模型组小鼠灌胃等量生理盐水。在给药的第7 d和第14 d测定各组小鼠的空腹血糖、体重、低密度脂蛋白（Low-density lipoprotein，LDL）、甘油三酯（Triglyceride，TG）、总胆固醇（Total cholesterol，TC）和高密度脂蛋白（High-density and lipoprotein，HDL）含量等。同时采用苏木精-伊红染色法检测小鼠胰腺组织病理学变化。结果表明，与正常组相比，模型组小鼠的体重和血糖含量均有极显著性变化（P<0.01），血清中TG、TC和LDL的含量均极显著性降低（P<0.01）；胰腺组织损伤严重，形态不完整，有明显的空泡产生。与模型组相比，200 mg/kg牦牛胶原蛋白肽可以显著改善糖尿病小鼠的症状，缓解糖尿病小鼠血糖及其它血液指标的增高趋势和胰腺组织的损伤程度。研究表明牦牛胶原蛋白肽可以显著性下调糖尿病小鼠的血糖水平，缓解糖尿病的症状。

Study of Yak Collagen Peptides on Reducing Blood Sugar

The effect of yak collagen peptide on reducing blood sugar was studied by diabetes mice model. A diabetic mouse model was prepared by intraperitoneal injection of alloxan (200 mg/kg) into Kunming mice. The modeled mice were randomly divided into normal group, model group, positive control group (metformin 200 mg/kg) and yak collagen peptide group (200 mg/kg), with 6 mice in each group. Relevant medicines were intragastric administration, given to each group once a day for consecutive 2 weeks, and normal group and model group were given constant volume of normal saline. The blood glucose, body weight, low-density lipoprotein (LDL), triglyceride (TG), total cholesterol (TC) and high-density lipoprotein (HDL) content of each group were measured after 7 and 14 days of administration. At the same time, pancreatic morphology of mice were detected by HE staining. Compared with the normal group, amount of body weight change and blood sugar content of the model group were significantly different (P<0.01). The TG, TC, and LDL levels of serum were all significantly reduced (P<0.01). The pancreatic tissue was severely damaged with incomplete morphology and obvious vacuoles. Compared with the model group, treatment of 200 mg/kg yak collagen peptides could significantly improve the symptoms of diabetic mice, alleviate the increasing tendency of blood sugar and other blood indicators and pancreatic damage in diabetic mice. The results showed that yak collagen peptide could obviously down-regulate the blood glucose level of diabetic mice, relieve symptoms of diabetes.