综合果蔬酵素发酵工艺优化及其润肠通便功能评价

目的：确定综合果蔬酵素发酵工艺，并探究其润肠通便功能。方法：通过调整发酵时间、发酵温度、低聚异麦芽糖添加量、燕麦麸皮添加量，以感官评分为评价指标，优化综合果蔬酵素的制备工艺，测定酵素产品的特征性成分，建立小鼠便秘模型，探究综合果蔬酵素不同剂量（低剂量5 mL/kg，中剂量10 mL/kg，高剂量15 mL/kg）对小鼠小肠墨汁推进率和排便的影响。结果：应用响应面法优化出综合果蔬酵素的配方为发酵时间为72 h，发酵温度为37℃，低聚异麦芽糖添加量为3%，燕麦麸皮添加量为3%，在此条件下感官评分为95.33分。特征性成分检测中综合果蔬酵素中富含1100 mg/kg乳酸、γ-氨基丁酸100 mg/kg、120 mg/100 g的游离氨基酸，小鼠试验中，高剂量的综合果蔬酵素的墨汁推进率高达66.7%，与对照组相比极显著（P<0.01）提升，6 h内排便粒数高剂量组达到33.16粒，极显著（P<0.01）高于模型对照组，有效缩短了粪便在小肠内的滞留时间，起到润肠通便的作用。结论：优化后的综合果蔬酵素具有较好的感官评分和润肠通便作用，具有一定的应用前景。

Optimization of Fermentation Technology of Integrated Fruit and Vegetable Enzyme and Evaluation of Its Function of Nourishing Bowel and Defecating

Objective: To determine the fermentation technology of dietary fiber enzyme, and to explore its function of nourishing intestine and defecating. Methods: By adjusting the fermentation time, fermentation temperature, the amount of isomaltose and oat bran, the sensory score of integrated fruit and vegetable enzyme was optimized, the characteristic components of the enzyme products were determined. To establish a constipation model in mice, and to explore the effects of different doses of Integrated fruit and vegetable enzymes (low dose 5 mL/kg, medium dose 10 mL/kg, high dose 15 mL/kg) on small intestinal ink propulsion rate and defecation in mice. Results: The formula of integrated fruit and vegetable enzyme was optimized by response surface method. The fermentation time was 72 h, the fermentation temperature was 37 ℃, the amount of isomaltose added was 3%, and the amount of oat bran added was 3%. In the mouse experiment, the ink advance rate of high-dose integrated fruit and vegetable enzyme was as high as 66.7%, compared with the control group, it was significantly improved (P<0.01), and the number of defecation grains within 6 h reached 33.16 in the high-dose group, it was significantly higher than the model control group (P<0.01) , which effectively shortened the retention time of feces in the small intestine, and played the role of moistening the intestine. Conclusion: The optimized integrated fruit and vegetable enzyme of fruits and vegetables has better sensory score and nourishing bowel function, so it has certain application prospect.