| 基于CoOOH纳米片氧化酶活性的比率荧光传感器检测抗坏血酸 |
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| 引用本文： | 贾宝珠,蔡美玲,邱芷靖,袁钰佩,何镇熹,华夏,王碧蔓,王弘,徐振林,吴民富,罗林. 基于CoOOH纳米片氧化酶活性的比率荧光传感器检测抗坏血酸[J]. 食品工业科技, 2022, 43(8): 273-280. DOI: 10.13386/j.issn1002-0306.2021070003 |
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| 作者姓名： | 贾宝珠 蔡美玲 邱芷靖 袁钰佩 何镇熹 华夏 王碧蔓 王弘 徐振林 吴民富 罗林 |
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| 作者单位： | 1.广东第二师范学院生物与食品工程学院，广东广州 5103032.广东省食品质量安全重点实验室，华南农业大学食品学院，广东广州 5106423.佛山职业技术学院食品科学系，广东佛山 528137 |
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| 基金项目： | 国家自然科学基金（31801621）；广东省重点领域研发计划项目（2019B020211002）；广东省自然科学基金（2021A1515012422）；广东省基础与应用基础研究基金省市联合基金（2020A1515110332）；广州市科技计划（202102021002）；广东大学生科技创新培育专项资金“攀登计划”项目（pdjh2021b0368，pdjih2020b0428）；华南农业大学大学生创新创业训练计划项目（202110564008，202110564011）。 |
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| 摘 要： | 研究建立一种基于羟基氧化钴纳米片(CoOOH)类氧化酶活性的比率荧光传感器,可快速、灵敏、准确地测定抗坏血酸(Ascorbic acid,AA).在氢氧化钠溶液中,以氯化钴为原料、次氯酸钠为氧化剂,在超声条件下制备具有类氧化酶活性的CoOOH纳米片,并采用透射电子显微镜(TEM)、紫外可见吸收光谱(UV-vis)、X射...
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| 关 键 词： | 抗坏血酸 羟基氧化钴纳米片 比率荧光传感器 |
| 收稿时间： | 2021-07-02 |
Development of a Ratiometric Fluorescence Sensor for Ascorbic Acid Based on Oxidase-mimicking Activity of CoOOH Nanoflake |
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| JIA Baozhu,CAI Meiling,QIU Zhijing,YUAN Yupei,HE Zhenxi,HUA Xia,WANG Biman,WANG Hong,XU Zhenlin,WU Minfu,LUO Lin. Development of a Ratiometric Fluorescence Sensor for Ascorbic Acid Based on Oxidase-mimicking Activity of CoOOH Nanoflake[J]. Science and Technology of Food Industry, 2022, 43(8): 273-280. DOI: 10.13386/j.issn1002-0306.2021070003 |
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| Authors: | JIA Baozhu CAI Meiling QIU Zhijing YUAN Yupei HE Zhenxi HUA Xia WANG Biman WANG Hong XU Zhenlin WU Minfu LUO Lin |
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| Affiliation: | 1.College of Biological and Food Engineering, Guangdong University of Education, Guangzhou 510303, China2.Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food, South China Agricultural University, Guangzhou 510642, China3.Department of Food Science Foshan Polytechnic, Foshan 528137, China |
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| Abstract: | This work aimed at developing a ratiometric fluorescence sensor for sensitive and accurate detection of ascorbic acid (AA) based on oxidase-mimicking activity of CoOOH nanoflake. CoOOH nanoflake with oxidase-like activity was prepared by using CoCl2 as precursor, NaClO as oxidant through a sonication method. The as-synthesized CoOOH nanoflake was characterized by transmission electron microscopy (TEM), UV–vis absorption, X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectra. Control experiments were used to demonstrate the feasibility for constructing the radiometric fluorescent sensor using the as-synthesized CoOOH nanoflake. Single-factor experiments were conducted to optimized the optimal concentration of CoOOH nanoflake and incubation time for AA detection. The results showed that the as-synthesized CoOOH nanoflakes exhibited typical hexagonal plate shape, and other characterization results also were consistent with those in previous works. The optimal concentration of CoOOH nanoflake was 39.1 μmol/L, and the optimal incubation time was 25 min. Under the optimal conditions, it was found that F428/F568 linearly correlated with AA concentration from 0.5 to 10.0 μmol/L with correlation coefficients of 0.9965. The regression equation was y=0.79416x+0.37917 (R2=0.9965) with a limit of detection of 303 nmol/L (RSN=3). The recoveries from fruit juice beverage samples spiked with varying concentrations of AA were in the range of 88.0%~115.9%, and analytical results of the sensor were correlated well with those of national standard method of China. These results indicated that the ratiometric fluorescent sensor had excellent selectivity and accuracy for AA detection, creating a new avenue for rapid detection of AA. |
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