苦丁茶冬青多糖对小鼠脾脏淋巴细胞的免疫调节和抗氧化作用

本文通过体外培养小鼠脾脏淋巴细胞研究苦丁茶冬青多糖对正常小鼠脾脏淋巴细胞免疫调节和抗氧化作用的影响。测定了不同浓度的苦丁茶冬青多糖（polysaccharide from Ilex kudingcha C.J. Tseng）对脾脏淋巴细胞体外增殖能力、抗氧化酶（SOD、T-AOC、GSH-Px和CAT）活力、丙二醛（MDA）含量及炎性细胞因子（TNF-α、IFN-γ和IL-1β）分泌水平的影响。结果显示，小鼠脾脏淋巴细胞的增殖与苦丁茶冬青多糖浓度呈正相关，在80和160 μg/mL浓度时，吸光度值分别为0.38和0.41，与空白对照组（0.31）相比具有极显著差异（P<0.01）；SOD、T-AOC、GSH-Px和CAT活力随苦丁茶冬青多糖浓度的增加而增强，浓度为160 μg/mL时，抗氧化酶活力分别为4.54 U/mg蛋白、1.06 mmol/mg蛋白、799.01 mU/mg蛋白和806.85 U/mg蛋白，与空白对照组相比具有极显著性差异（P<0.01）；此外，与空白对照组相比，苦丁茶冬青多糖显著降低MDA含量、促进NO分泌和促进脾脏淋巴细胞分泌TNF-α、IFN-γ和IL-1β。本研究结果表明苦丁茶冬青多糖可通过促进小鼠脾脏淋巴细胞增殖、增强细胞抗氧化能力及诱导炎性细胞因子分泌等发挥其免疫增强活性，该研究可为苦丁茶冬青的开发和利用提供理论支撑。

Effect of Polysaccharide from Ilex kudingcha C.J. Tseng on the Immune Regulation and Antioxidation of Spleen Lymphocytes from Mice

To investigate the immune regulation and antioxidation effects of polysaccharide from Ilex kudingcha C.J. Tseng on mouse spleen lymphocytes in vitro. The proliferation, antioxidant capacity (SOD, T-AOC, GSH-Px and CAT) activity and malonic dialdehyde (MDA) content, as well as the secretion of cytokines (TNF-α, IFN-γ and IL-1β) were detected after treating with polysaccharide from Ilex kudingcha C.J. Tseng. The results showed that polysaccharide from Ilex kudingcha C.J. Tseng could promote the proliferation of spleen lymphocytes dose-dependently. Compared to the OD value of control group (0.31), the OD values at the concentration of 80 and 160 μg/mL were increased significantly (P<0.01) to 0.38 and 0.41, respectively. The activities of SOD, T-AOC, GSH-Px and CAT were increased with increasing concentration of polysaccharide from Ilex kudingcha C.J. Tseng. And there were significant differences (P<0.01) between control group and 160 μg/mL polysaccharide from Ilex kudingcha C.J. Tseng group, the activities of SOD, T-AOC, GSH-Px and CAT were of 4.54 U/mg protein, 1.06 mmol/mg protein, 799.01 mU/mg protein and 806.85 U/mg protein, respectively. Meanwhile, compared to control group, polysaccharide from Ilex kudingcha C.J. Tseng could significantly decrease the contents of MDA, increase NO production and promote the secretion of TNF-α, IFN-γ and IL-1β. In conclusion, the immunomodulatory effect of polysaccharide from Ilex kudingcha C.J. Tseng may be related to the promotion of splenic lymphocyte proliferation, the enhancement of antioxidant activity and the induction of cytokine secretion level. The results are beneficial to provide a theoretical basis for the development and utilization of Ilex kudingcha C.J. Tseng.