硫酸化修饰对小球藻胞内多糖抗氧化及抗肿瘤活性的影响

为探究小球藻多糖硫酸化修饰对生物活性影响,本研究采用小球藻(Chlorella sp.22)为实验材料,提取胞内多糖经DEAE-52纤维素阴离子交换柱分离纯化得到纯化多糖HDB-1,对纯化多糖HDB-1进行硫酸化修饰得SHDB-1,对硫酸化修饰前后多糖进行抗氧化活性和抗肿瘤活性研究。结果表明,纯化组分HDB-1以α-呋喃糖为主。用三氧化硫-吡啶法对HDB-1进行硫酸化修饰得到SHDB-1,其取代度为1.046。抗氧化活性结果表明,HDB-1与SHDB-1的DPPH自由基清除率IC₅₀(半抑制浓度)值分别为29.28 mg/mL和14.49 mg/mL,羟自由基清除率IC₅₀值分别为36.75 mg/mL和28.59 mg/mL,可知SHDB-1抗氧化效果优于HDB-1。抗肿瘤结果表明,HDB-1及SHDB-1在1 mg/mL浓度下对细胞无毒性,HDB-1与SHDB-1的IC₅₀值分别为960.16μg/mL及658.19μg/mL,可知SHDB-1抑制宫颈癌细胞Hela增殖效果优于HDB-1。以上结果表明,硫酸化修饰的小...

Effect of Sulfation Modification on Antioxidation and Antitumor Activity of Intracellular Polysaccharide from Chlorella vulgaris

In order to explore the effect of Chlorella polysaccharide sulfation modification on biological activity, Chlorella sp.22 was used as experimental material in this study. The purified polysaccharide HDB-1 was obtained by extracting intracellular polysaccharides and separated and purified by DEAE-52 cellulose anion exchange column. The purified polysaccharide HDB-1 was sulfated to obtain SHDB-1. The antioxidant activity and antitumor activity of polysaccharides before and after sulfation modification were studied. The results showed that the purified component HDB-1 was mainly α-furanose. SHDB-1 was obtained by sulfation modification of HDB-1 by sulfur trioxide-pyridine method. Its degree of substitution was 1.046. The antioxidant activity results showed that the IC₅₀ (semi-inhibitory concentration) values of HDB-1 and SHDB-1 DPPH radical clearance were 29.28 mg/mL and 14.49 mg/mL, respectively. The IC₅₀ values of hydroxyl radical scavenging were 36.75 mg/mL and 28.59 mg/mL, respectively, indicating that the antioxidant effect of SHDB-1 was better than that of HDB-1. The antitumor results showed that HDB-1 and SHDB-1 were not toxic to cells at 1 mg/mL concentrations. The IC₅₀ values of HDB-1 and SHDB-1 were 960.16 μg/mL and 658.19 μg/mL, respectively, indicating that SHDB-1 inhibited the proliferation of Hela in cervical cancer cells better than HDB-1. The above results show that compared with HDB-1, the antioxidant activity and in vitro inhibition of cancer cell proliferation of the sulfation-modified Chlorella intracellular polysaccharides SHDB-1 are significantly improved.