菠萝蜜多糖对小鼠肠道菌群的调节作用

为探究菠萝蜜多糖（JFP-Ps）对小鼠肠道菌群多样性及其结构的影响，将24只雄性昆明小鼠随机分为四组:对照组、JFP-Ps（50 mg/kg BW）组、JFP-Ps（100 mg/kg BW）组和JFP-Ps（200 mg/kg BW）组，连续灌喂2周后，收集新鲜小鼠粪便，提取粪便DNA扩增肠道菌群16S rRNA基因的V3~V4区，通过Illumina高通量测序技术研究菠萝蜜多糖对小鼠肠道菌群的调节作用。结果表明，JFP-Ps可通过调节拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）等肠道优势菌群的组成和结构，增加肠道菌群的多样性。通过属水平分析发现，JFP-Ps可增加拟杆菌属（Bacteroides）、异杆菌属（Allobaculum）、乳酸杆菌属（Lactobacillus）和副拟杆菌（Parabacteroides）等产SCFAs菌属的丰度，减少普雷沃菌属（Prevotella）等菌属丰度，发挥肠道微生物调节作用，改善宿主肠道微生态健康，研究结果为JFP-Ps在肠道益生产品的研发应用奠定理论基础。

Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp on Gut Microbiota in Mice

The present study aimed to investigate the modulatory effect of polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on the diversity and structure of gut microbiota in mice. 24 male Kunming mice were randomly divided into four groups: Control group, JFP-Ps (50 mg/kg BW) group, JFP-Ps (100 mg/kg BW) group and JFP-Ps (200 mg/kg BW) group. The colonic feces of mice were collected after treating with JFP-Ps in different doses for 2 weeks. Then the DNA was extracted and the V3~V4 region of the 16S rRNA gene of the intestinal flora was amplified. The regulatory effect of JFP-Ps on the gut microbiota of mice was studied using Illumina Miseq high-throughput sequencing technology. The results showed that Bacteroidetes and Firmicutes were the dominant intestinal flora, and JFP-Ps modulated the composition of Bacteroidetes and Firmicutes in comparison with the control group. At the genus level, the abundance of SCFAs-producing bacteria, including Bacteroides, Allobaculum, Lactobacillus and Parabacteroides were significantly increased in response to JFP-Ps treatment, but a significant decrease in Prevotella. These results indicated that JFP-Ps could improve the diversity of gut microbiota by regulating the composition and structure of dominant bacteria such as Bacteroidetes and Firmicutes. This study would provide a theoretical basis for JFP-Ps in intestinal prebiotics.