英国红芸豆抗氧化肽组分对H2O2诱导PC12细胞氧化应激损伤的保护作用

为了探究英国红芸豆抗氧化肽组分的抗氧化作用，采用超滤、葡聚糖凝胶G-15层析技术对碱性蛋白酶酶解英国红芸豆抗氧化蛋白质制备的抗氧化产物进行分级，研究抗氧化活性较高的组分对H₂O₂损伤的PC12细胞的影响。结果表明:抗氧化肽组分BRKBAPC-2能缓解H₂O₂对PC12造成的细胞生长抑制作用，随着肽浓度的提高，细胞内活性氧（ROS）水平，丙二醛（MDA）含量及乳酸脱氢酶（LDH）胞外活力均有所降低，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活力以及还原型谷胱甘肽（GSH）含量均具有所提高，尤其BRKBAPC-2浓度为200 μg/mL时，ROS水平为32.53%，MDA含量为0.96 nmol/μg prot，LDH胞外活力为202.12 U/L，与模型组相比，均极显著降低（P<0.01）；SOD活力为（555.48±3.64）U/mg prot，CAT活力为（14.77±1.30）U/mL，GSH含量为（140.88±8.19）μmol/g prot，与模型组相比，均极显著提高（P<0.01），此外，其可抑制线粒体膜电位的降低，改善细胞周期的阻滞情况，阻止细胞凋亡关键酶Caspase-3和Caspase-9的激活。可见，抗氧化肽组分BRKBAPC-2对H₂O₂引起的PC12细胞氧化损伤具有一定的保护作用。

Protective Effects of British Red Kidney Bean Antioxidant Peptide Components on H2O2 Induced Oxidative Stress Damage in PC12 Cells

In order to explore the antioxidant effect of the British red kidney bean antioxidant peptide components, ultrafiltration and dextran gel G-15 chromatography technology were used to classify the antioxidant products prepared by alkaline protease enzymatic hydrolysis of the British red kidney bean antioxidant protein, and the effect of components with higher antioxidant activity on PC12 cells damaged by H₂O₂ was studied. The results showed that the antioxidant peptide component BRKBAPC-2 could alleviate the cell growth inhibitory effect of H₂O₂ on PC12. With the increasing of peptide concentration, the level of intracellular reactive oxygen species (ROS) levels, malondialdehyde (MDA) content and lactate dehydrogenase (LDH) extracellular activity were all reduced, the activity of superoxide dismutase (SOD), catalase (CAT) and the content of reduced glutathione (GSH) were all increased, especially when the concentration of BRKBAPC-2 was 200 μg/mL, ROS level was 32.53%, MDA content was 0.96 nmol/μg prot, LDH extracellular activity was 202.12 U/L, compared with the model group, they were significantly reduced (P<0.01). SOD activity was (555.48±3.64) U/mg prot, CAT activity was (14.77±1.30) U/mL, and GSH content was (140.88±8.19) μmol/g prot, compared with the model group, they were significantly improved (P<0.01). In addition, the BRKBAPC-2 with 200 μg/mL could inhibite the decrease of mitochondrial membrane potential, improve cell cycle resistance, prevent the activation of the key enzymes Caspase-3 and Caspase-9 of cell apoptosis. It could be seen that the antioxidant peptide component BRKBAPC-2 had a certain protective effect on the oxidative damage of PC12 cells caused by H₂O₂.