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黑果腺肋花楸叶黄酮的提取工艺优化及抗氧化、结合胆酸盐能力分析
引用本文:何旭华,石志娇,王安娜,赵春芳,刘云,阚欢.黑果腺肋花楸叶黄酮的提取工艺优化及抗氧化、结合胆酸盐能力分析[J].食品工业科技,2023,44(2):253-260.
作者姓名:何旭华  石志娇  王安娜  赵春芳  刘云  阚欢
作者单位:西南林业大学生命科学学院,云南昆明 650224
摘    要:以超声波辅助法提取黑果腺肋花楸叶黄酮(Flavonoids from Aronia melanocarpas’ leaves,AMF),探究其体外抗氧化活性和胆酸盐结合能力。以黄酮得率为指标,在单因素(液料比、乙醇浓度、超声功率、超声时间)的基础上,结合响应面法,优化AMF的提取工艺,并以DPPH·、ABTS+·清除率及总还原能力为指标,考察AMF的抗氧化活性;以胆酸钠、牛黄胆酸钠、甘氨胆酸钠为结合对象,考察AMF的胆酸盐结合能力。结果表明,AMF的最优提取工艺为:液料比61:1 mL/g、乙醇浓度60%、超声功率100 W、超声时间59 min,黄酮得率为24.22%±0.29%,与模型预测值(24.27%)接近。最优工艺制备的AMF对DPPH·、ABTS+·清除率较强,其IC50值分别为0.32、0.16 mg/mL;质量浓度为0.5 mg/mL时,总还原能力较好,其吸光值为1.09;AMF对胆酸钠、牛黄胆酸钠、甘氨胆酸钠具有较强结合能力,结合率的IC50值分别为0.76、3.01、6.49 m...

关 键 词:黑果腺肋花楸叶  黄酮  超声波辅助提取  工艺优化  抗氧化能力  结合胆酸盐能力
收稿时间:2022-04-27

Optimization of Extraction Process of Flavonoids from Aronia melanocarpas' Leaves and Analysis of Their Antioxidant and Bile Salt Binding Capacity
HE Xuhua,SHI Zhijiao,WANG Anna,ZHAO Chunfang,LIU Yun,KAN Huan.Optimization of Extraction Process of Flavonoids from Aronia melanocarpas' Leaves and Analysis of Their Antioxidant and Bile Salt Binding Capacity[J].Science and Technology of Food Industry,2023,44(2):253-260.
Authors:HE Xuhua  SHI Zhijiao  WANG Anna  ZHAO Chunfang  LIU Yun  KAN Huan
Affiliation:College of Life Sciences, Southwest Forestry University, Kunming 650224, China
Abstract:In this study, the antioxidant and bile salt binding activities of flavonoids from Aronia melanocarpas' leaves (AMF) by ultrasound-assisted extraction. Based on single factors (liquid-solid ratio, ethanol concentration, ultrasonic power and ultrasonic time) and response surface method, the extraction process of AMF was optimized with flavonoid yield as the index. The antioxidant activity of AMF was evaluated by DPPH·, ABTS+· scavenging rate and reduction power. The bile salt binding ability of AMF was investigated by using sodium cholate, sodium taurocholate and sodium glycocholate as binding objects. The results showed that the optimal extraction conditions were as follows: The liquid-solid ratio of 61:1 mL/g, the ethanol concentration of 60%, the ultrasonic power of 100 W, and the ultrasonic time of 59 min. Under the optimized conditions, the yield of flavonoids was 24.22%±0.29%, which approximated the predicted value (24.27%). The IC50 of DPPH and ABTS+ radicals scavenging capacities of AMF were 0.32 and 0.16 mg/mL, respectively. The AMF demonstrated the strongest reducing power with an absorbance value of 1.09 at the sample concentration of 0.5 mg/mL. The IC50 of AMF for the binding rate of sodium cholate, sodium taurocholate and sodium glycocholate were 0.76, 3.01 and 6.49 mg/mL, respectively. In summary, AMF exhibited strong antioxidant and bile salt binding capacity. This study could provide a basis for AMF to further develop natural antioxidants and hypolipidemic drugs.
Keywords:
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