低共熔溶剂提取江南卷柏穗花杉双黄酮的工艺优化

采用低共熔溶剂超声辅助技术提取江南卷柏中穗花杉双黄酮(AME），并优化其工艺参数。通过一步法制备3种低共熔溶剂，并筛选出提取江南卷柏AME效果最佳低共熔溶剂，通过核磁共振、傅里叶红外光谱和热重分析表明低共熔溶剂被成功制备，并采用单因素和响应面法优化江南卷柏中AME的提取工艺。结果表明，最佳低共熔溶剂为氯化胆碱:香叶醇，最佳提取工艺条件为:摩尔比1:2，含水量20%，液固比10 mL/g，超声时间31 min，超声功率为240 W。在此条件下，AME的提取得率为（2.75±0.12） mg/g。与热浸法、冷浸法、离子液体超声提取法、乙醇超声提取法进行对比表明，低共熔溶剂结合超声提取不仅能够节约提取时间，还能显著增加AME提取得率。

Deep Eutectic Solvents Extraction and Optimization of Amentoflavone from Selaginella moellendorffii

To extract the amentoflavone (AME) from Selaginella moellendorffii with deep eutectic solvent technology, three kinds of deep eutectic solvents were prepared by one-step method, and the best deep eutectic solvent for extracting AME of Selaginella moellendorffii was selected. The results of the clear magnetic resonance, fourier infrared spectroscopy and thermogravimetric analysis indicated that the deep eutectic solvent was successfully prepared. The extraction process of AME from Selaginella moellendorffii was optimized by single factors and response surface method. Results showed that, the best deep eutectic solvent was choline chloride:geraniol. The best extraction conditions were molar ratio 1:2, water content 20%, liquid-solid ratio 10 mL/g, ultrasound time 31 min, and the ultrasonic power was 240 W. Under this condition, the actual content of AME was (2.75±0.12) mg/g. The results of comparison with hot immersion method, cold immersion method, ionic liquid ultrasonic extraction method and ethanol ultrasonic extraction method showed that the deep eutectic solvent combined with ultrasonic extraction could not only save the extraction time, but also significantly increase the AME extraction rate.