青海管花肉苁蓉不同部位的功能性成分及其抗氧化活性

本文以青海地区仿野生栽培的管花肉苁蓉为原料，对其不同部位生物活性物质以及抗氧化活性进行比较分析。结果表明，肉苁蓉不同部位的多种有效成分差异显著，根部总多酚、总多糖、总三萜、松果菊苷和毛蕊花糖苷的含量显著高于中部和顶部（P<0.05）。其中松果菊苷与毛蕊花糖苷总含量为4.67%，满足2020版《中国药典》的规定。肉苁蓉不同部位的抗氧化能力强弱顺序为根部>中部>顶部，根、中、顶三部位对ABTS自由基清除能力的IC₅₀值分别为0.91、1.59、2.32 mg/mL，对DPPH自由基清除能力的IC₅₀值分别为0.77、5.16、10.66 mg/mL。相关性分析表明，肉苁蓉不同部位抗氧化活性与多种生物活性物质含量密切相关，其ABTS IC₅₀值、DPPH IC₅₀值与总多酚、总三萜、总多糖、松果菊苷以及毛蕊花糖苷含量均呈负相关关系，且相关系数较高。本文为管花肉苁蓉的药、食安全以及综合开发利用提供了理论参考。

Bioactive Components and Antioxidant Activities of Different Parts of Cistanche tubulosa in Qinghai Province

In this paper, the bioactive components and antioxidant activities of Cistanche tubulosa (CT) cultivated in Qinghai Province were comprehensively analyzed. The results showed that the bioactive components in different parts of Cistanche tubulosa were significantly different (P<0.05). Contents of total polyphenols, total polysaccharides, total triterpenes, echinoside and verbascoside in bottom part were significantly higher than those in the middle and top parts. Especially, the contents of the total echinoside and verbascoside content was 4.67%, which was higher than the standard of Chinese pharmacopoeia 2020 edition. The IC₅₀ values of ABTS free radical scavenging abilities of root, middle and top parts were 0.91, 1.59, 2.32 mg/mL, respectively. The IC₅₀ values of DPPH free radical scavenging activities were 0.77, 5.16, 10.66 mg/mL, respectively. The antioxidant activities of different parts of Cistanche tubulosa were closely related to the contents of various bioactive substances, the IC₅₀ value of ABTS and DPPH were negatively correlated with the contents of total polyphenols, total triterpenoids, total polysaccharides, echinoside and verbascoside, and the correlation coefficient was high. The results will provide a theoretical basis for the medicine, food safety and utilization of Cistanche tubulosa in Qinghai Province.