| 兔肝金属硫蛋白制备中试工艺研究 |
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| 引用本文： | 赵新民,周攀登,汤青云,曹醴承. 兔肝金属硫蛋白制备中试工艺研究[J]. 广东化工, 2011, 38(12): 187-188 |
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| 作者姓名： | 赵新民 周攀登 汤青云 曹醴承 |
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| 作者单位： | 1. 湖南城市学院化学与环境工程系,湖南益阳,413000
2. 湖南麓谷生物技术有限公司,湖南长沙,415000 |
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| 摘 要： | 以硫酸锌诱导家兔肝组织为原料,采取超滤、凝胶层析和离子交换层析方法对金属硫蛋白精品制备进行3批中试试验。结果表明,金属硫蛋白平均含量为96.33%,平均收率为2.762.65 mg/g,;金属含量为6.59%;含水量小于1.71%。说明中试工艺条件是合理的。试验为金属硫蛋白进一步产业化提供依据。
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| 关 键 词： | 锌金属硫蛋白 兔肝 中试 |
Pilot-scale Study on the Preparation of Metallothionein from Rabbit Livers |
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| Zhao Xinmin,Zhou Pandeng,Tang Qingyun,Cao Licheng. Pilot-scale Study on the Preparation of Metallothionein from Rabbit Livers[J]. Guangdong Chemical Industry, 2011, 38(12): 187-188 |
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| Authors: | Zhao Xinmin Zhou Pandeng Tang Qingyun Cao Licheng |
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| Affiliation: | 1.Department of Chemistry and Environmental Engineering,Hunan City University,Yiyang 413000; 2.Hunan Lugubiotechnology Company,Changsha 415000,China) |
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| Abstract: | Pilot-scale preparation of metallothionein from rabbit liver was carried out using Sephadex G-75 gel filtration chromatography and DEAE chromatography.The results showed that the average content metallothionein,Zn,water and percent yield were 96.33 %,6.59 %,1.71 % and 2.762.65 mg/g（wet tissue） respectively.Thus,it proved that the pilot-scale process parameters were resonable and it could be used asreference for industrios preparation of metallothionein. |
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| Keywords: | metallothionein rabbit liver pilot-scale |
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