生物表面活性剂鼠李糖脂发酵条件的优化及绿色提取新工艺研究

通过正交实验对铜绿假单胞杆菌产鼠李糖脂（RL）的发酵条件进行了优化,并采用预处理酸沉淀冷冻干燥法新工艺提取RL。在最优发酵条件下RL的产量可达56 g/L以上。提取后的RL由二鼠李糖脂和单鼠李糖脂两种同系物组成,可将去离子水的表面张力降至29.01 mN/m。该RL提取工艺是一项绿色工艺。

Study on Green Extraction Procedures and Optimization on Fermentation Conditions of Biosurfactant Rhamnolipids

The optimized conditions for fermentation of rhamnolipids（RL） produced by pseudomonas aeruginosa were studied by orthogonally designed experiments.RL was extracted through new procedures of pretreatment,acid precipitation followed by freeze-dryness.The experiments indicated that RL yield was 56 g/L and more under the optimal conditions.Dirhamnolipid and monorhamnolipid were identified from the extracted RL that could decrease the surface tension of deionized water to 29.01 mN/m.The extraction procedures is a green technique.