| 胃蛋白酶提取硫酸软骨素的研究 |
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| 引用本文： | 陈红丽,杨永锋,于建军.胃蛋白酶提取硫酸软骨素的研究[J].应用化工,2009,38(12):1766-1768. |
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| 作者姓名： | 陈红丽 杨永锋 于建军 |
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| 作者单位： | 河南农业大学,烟草学院,河南,郑州,450002 |
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| 摘 要： | 以鸡胸软骨为原料,采用稀碱-双酶(胰蛋白酶和胃蛋白酶)相结合的方法提取硫酸软骨素。研究了胃蛋白酶水解硫酸软骨素的影响因素,结果显示,胃蛋白酶水解部分pH值为5.9,酶解时间为100 m in,料液比为1 000∶1.1(g/mL),酶解温度为40℃,在此条件下,硫酸软骨素产率达到25.80%,纯度为85.65%,质量有较大提高。
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| 关 键 词： | 硫酸软骨素 胃蛋白酶 纯度 |
Research on stomach proteinase extracting chondroitin sulfate |
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| CHEN Hong-li,YANG Yong-feng,YU Jian-jun.Research on stomach proteinase extracting chondroitin sulfate[J].Applied chemical industry,2009,38(12):1766-1768. |
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| Authors: | CHEN Hong-li YANG Yong-feng YU Jian-jun |
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| Abstract: | The method of dilute alkali-enzyme hydrolysis to extract chondroitin sulfate from the chicken breast cartilage was studied.The effect of stomach proteinase on the production purity of the CS was studied.The results showed the best reaction conditions:pH value of stomach proteinase 5.9,enzymolysis time 100 min,ratio of filter liquor to enzyme 1 000 ∶ 1.1(g/mL),enzymolysis temperature 40 ℃.Under the conditions,the highest production rate was 25.80% and production purity was 85.65%,its quality was improved better. |
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| Keywords: | chondroitin sulfate stomach proteinase purity |
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