| HSV-tk联合GCV自杀系统对肝癌细胞株Huh-7杀伤作用的研究 |
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| 引用本文： | 郭国英,谢国良,何国清,孔彦平,王毅刚. HSV-tk联合GCV自杀系统对肝癌细胞株Huh-7杀伤作用的研究[J]. 浙江理工大学学报, 2011, 28(2) |
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| 作者姓名： | 郭国英 谢国良 何国清 孔彦平 王毅刚 |
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| 作者单位： | 浙江理工大学生命科学学院新元医学与生物技术研究所,杭州,310018 |
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| 基金项目： | 国家自然科学基金，浙江省"生物医学工程"重中之重学科开放基金 |
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| 摘 要： | 单纯疱疹病毒胸苷激酶(herpessimplex virus-thymidine kinase,HSV-tk)基因,是目前研究比较多的自杀基因,其与前体药物丙氧鸟苷(ganciclovir,GCV)构成HSV-tk/GCV自杀基因系统.本实验将携带HSV-tk基因的慢病毒转染Huh-7细胞,通过报告基因反映慢病毒的转染效率;基因组PCR及RT-PCR检测转染Huh-7细胞中的HSV-tk基因的整合及表达;MTT检测不同浓度药物处理后的细胞存活率;最后HSV-tk+与HSV-tk-的Huh-7细胞以不同比例混合,MTT测定GCV作用后细胞的存活率.结果表明,HSV-tk/GCV系统作用于Huh-7细胞后,细胞出现明显的凋亡现象,旁观者效应比较明显.
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| 关 键 词： | 肝癌细胞 Huh-7 旁观者效应 |
The Killing Effects of HSV-tk/GCV Suicide System on Hepatocellular Cell Line Huh-7 |
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| GUO Guo-ying,XIE Guo-liang,HE Guo-qing,KONG Yan-ping,WANG Yi-gang. The Killing Effects of HSV-tk/GCV Suicide System on Hepatocellular Cell Line Huh-7[J]. Journal of Zhejiang Sci-tech University, 2011, 28(2) |
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| Authors: | GUO Guo-ying XIE Guo-liang HE Guo-qing KONG Yan-ping WANG Yi-gang |
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| Affiliation: | GUO Guo-ying,XIE Guo-liang,HE Guo-qing,KONG Yan-ping,WANG Yi-gang(Xinyuan Institute of Medicine and Biotechnology,School of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China) |
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| Abstract: | Herpes simplex virus thymidine kinase(HSV-tk) gene/ganciclovir(GCV) has been wildly used as a traditional gene therapy modality,This study explores the killing effects of lenteviral vector carried HSV-tk/GCV suiside system to Huh-7 cell line.First of all,Flurescence imaging assay detects the transfection efficiency of lenti virus,HSV-tk expression is examined by Genome PCR and RT-PCR;Then,MTT assay tested the growth inhibition effects of different concentrations of GCV on HSV-TK+Huh-7 cell line and various ... |
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| Keywords: | HSV-tk GCV |
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