| GSK-3抑制剂增强携带TRAIL的溶瘤腺病毒对肝癌细胞的杀伤作用 |
| |
| 引用本文： | 谢国良,肖连立,李新,何国清,陈磊,席璐,章康健,王毅刚.GSK-3抑制剂增强携带TRAIL的溶瘤腺病毒对肝癌细胞的杀伤作用[J].浙江理工大学学报,2011,28(2). |
| |
| 作者姓名： | 谢国良 肖连立 李新 何国清 陈磊 席璐 章康健 王毅刚 |
| |
| 作者单位： | 浙江理工大学生命科学院新元医学与生物技术研究所,杭州,310018 |
| |
| 基金项目： | 国家自然科学基金，浙江省"生物医学工程"重中之重学科开放基金 |
| |
| 摘 要： | 研究携带TRAIL基因的溶瘤腺病毒联合化疗药物GSK-3抑制剂(氯化锂和SB-415286)对人肝癌细胞的体外杀伤作用.采用MTT法检测病毒ZD55-TRAIL联合化疗药物氯化锂和SB-415286对肝癌细胞株HepG-2、BEL-7404以及正常细胞株L02、QSG-7701增殖的抑制作用,并通过结晶紫实验检测进一步证明联合用药的杀伤作用和安全性;利用Hoechst33342染色对肝癌细胞株BEL-7404的细胞凋亡进行形态学观察;通过Western blot检测肝癌细胞HepG-2细胞凋亡信号通路的变化.结果表明:低剂量的氯化锂和SB-415286能显著提高ZD55-TRAIL对肝癌细胞株HepG-2、BEL-7404的杀伤作用,对人体肝正常细胞株L02、QSG-7701无明显的抑制作用.说明GSK-3抑制剂能够解除肝癌细胞对TRAIL的抗性,增强携带TRAIL基因的溶瘤腺病毒对肝癌细胞的杀伤.
|
| 关 键 词： | 溶瘤腺病毒 氯化锂 联合抑癌 |
Enhanced Killing Effects on Hepatoma Cells by TRAIL Armed Oncolytic Adenovirus and Glycogen Synthase Kinase-3 Inhibitors |
| |
| XIE Guo-liang,XIAO Lian-li,LI Xin,HE Guo-qing,CHEN Lei,XI Lu,ZHANG Kang-jian,WANG Yi-gang.Enhanced Killing Effects on Hepatoma Cells by TRAIL Armed Oncolytic Adenovirus and Glycogen Synthase Kinase-3 Inhibitors[J].Journal of Zhejiang Sci-tech University,2011,28(2). |
| |
| Authors: | XIE Guo-liang XIAO Lian-li LI Xin HE Guo-qing CHEN Lei XI Lu ZHANG Kang-jian WANG Yi-gang |
| |
| Affiliation: | XIE Guo-liang,XIAO Lian-li,LI Xin,HE Guo-qing,CHEN Lei,XI Lu,ZHANG Kang-jian,WANG Yi-gang(Xinyuan Institute of Medicine and Biotechnology,School of Life Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China) |
| |
| Abstract: | The study explores the combinational killing effects of oncolytic adenovirus-mediated TRAIL and chemotherapeutic agent GSK-3 inhibitors(LiCl and SB-415286)against human hepatoma cells in vitro.First of all,MTT assay tests the growth inhibition effects of combinational therapy with LiCl/SB-415286 and ZD55-TRAIL on hepatocellular cell lines HepG-2,BEL-7404 and normal cell lines L02,QSG-7701;Further,the cytopathic effect assay demonstrates the efficacy and the safety of the combination therapy and the apoptosi... |
| |
| Keywords: | TRAIL SB-415286 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
