杏鲍菇超氧化物歧化酶部分酶学性质研究

研究了杏鲍菇不同部位超氧化物歧化酶（SOD）活力的差异,以及pH、温度、金属离子和有机试剂对SOD酶活性的影响。结果表明:菌盖和菌褶SOD酶活力显著高于菌柄,外皮高于心部;杏鲍菇SOD在p H5⁸范围内活性稳定,具有良好的耐热性;Cu²⁺、Zn²⁺、Fe²⁺和Mn²⁺对SOD酶活力具有不同程度的激活作用,其中Cu²⁺的激活作用最突出;甲醇、乙醇和正丁醇对SOD活性的影响均为低浓度激活、高浓度抑制,而丙酮则表现出抑制作用。可见杏鲍菇SOD酶活力受到p H、温度、金属离子和有机溶剂等多种因素的影响。 

Study on properties of superoxide dismutase activity from Pleurotus eryngii

Superoxide dismutase( SOD) activity in different parts of Pleurotus eryngii was compared.Several factors affecting the stability of SOD including p H,temperature,metal ions and organic reagents,were studied.The results showed that SOD enzyme activities in pileus and gill were higher than that in stipe,the skin higher than core section.This enzyme displayed good stability at the p H range from 5 to 8 and good thermal stability. Cu~(2+),Zn~(2+),Fe~(2+) and Mn~(2+) showed different degrees of activation on SOD enzyme activity,in which the activation of Cu~(2+) was the most prominent. Methanol,ethanol and n- butyl alcohol showed activation in low concentration but inhibitory effect in high concentration,while acetone showed inhibitory effect.Therefore SOD activity of Pleurotus eryngii were influenced by many factors including p H,temperature,metal ions and organic solvents.