神秘果种子多酚大孔树脂纯化工艺研究

分别采用6种大孔树脂(AB-8、D101、HPD-500、S-8、DM130、X-5)纯化神秘果种子多酚,以吸附量、吸附率、解吸量、解吸率为评定参数,优选出X-5为最佳树脂。研究其吸附等温线,发现其与Langmuir等温线拟合良好。静态吸附与解吸结果表明:最佳pH 5.8,最佳解吸液为70%乙醇。动态吸附与解吸结果表明:最优样品液浓度为1.2mg/mL,吸附液体积为100mL,解吸液体积为50mL,吸附流速为1.0mg/mL,解吸流速为1.5mg/mL。研究结果表明:经X-5纯化后的神秘果种子多酚含量提高了2倍,T-AOC测定总抗氧化能力提高了2.5倍。说明该工艺适用于神秘果种子多酚的纯化。

Purification of polyphenols from Synsepalum dulcificum seed with macroporous resins

Taking the absorbability and desorption properties as parameters, the purifying conditions of polyphenol from Synsepalum dulcificum seed by macroporous resins, compared with five types of macroporous resins were investigated. The result showed that the X-5 macroporous resins was the best one. Through a nonlinear curve fitting with the Langmuir adsorption models, it was found that the fitting degree of adsorption isotherm and Langmuir function curve was very high. The optimal adsorption process conditions were as followed: pH 5.8, 1.0 mL/min adsorption velocity. Besides, the best desorption conditions were: sample volume 100 mL, desorption velocity of flow 1.5 mL/min, dynamically elute with 50 mL concentration of desorption soluntion 70%. After being separated and purified by resin, the polyphenol content improved about 2 times and total antioxidant capacity significantly improved about 2.5 times. The method can be used for purification of polyphenols from Synsepalum dulcificum seed.